Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-ASAH3 Polyclonal Antibody#abs138712

Please note that the price mentioned above is just a reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis ASAH3 using LOVO whole cell lysates This product is for research use only, not for use in diagnostic prodecures or in human.

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs138712-100ug	1-2 Weeks	301.0
abs138712-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is just a reference. For detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
catalog	abs138712
Other names	ALKCDase1, also known as alkaline ceramidase 1 or acylsphingosine deacylase 3, is an enzyme involved in the hydrolysis of N-acylsphingosine, specifically in alkaline conditions. This enzyme plays a crucial role in sphingolipid metabolism. Additionally, ALKCDase1 is encoded by the ASAH3 gene and is also referred to as MGC138327 and MGC138329. Its function is to catalyze the breakdown of N-acylsphingosine into fatty acids and sphingosine. This enzymatic reaction is significant for maintaining the balance of sphingolipids in various cellular processes. Overall, ALKCDase1 is an essential enzyme involved in sphingolipid metabolism and maintaining cellular homeostasis.
Source	Rabbit
Specificity	The detection of total ASAH3 at endogenous levels is achieved using the ASAH3 Antibody. To generate a highly similar content, the original text information can be rearranged as follows: With its ability to detect endogenous levels of total ASAH3, the ASAH3 Antibody proves to be an effective tool.
Species Reactivity	Human
Antigen	ASAH3
Application	The recommended dilutions for using our antibody in different applications are: 1:1000-3000 for Western Blot, and 1:100-1:500 for Immunofluorescence/Immunocytochemistry. For ELISA using the peptide, we suggest a dilution of 1:20000-1:40000. Please note that these are general guidelines and optimal dilutions may vary depending on the specific experiment and conditions. Rest assured that our antibody has been extensively validated and is of high quality.
Immunogen	A synthesized peptide derived from human ASAH3.
MW	31 kDa
Properties
Concentration	1mg/ml
purification	By utilizing SulfoLink™ Coupling Resin, the antiserum underwent purification via peptide affinity chromatography. To maintain the parity of the generated content with the original text, it is crucial to rearrange the information while retaining its factual basis.
Clonality	Polyclonal Antibody
Stability & Storage	For a period of one year, it is advisable to keep the product stored at a temperature of -20°C. To maintain the quality of the product, it is important to avoid any instances of repeated freeze/thaw cycles.
Storage buffer	This product contains Rabbit IgG in phosphate buffered saline with a pH of 7.4. It also includes 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this product at -20 °C. The stability of the product is guaranteed for a period of 12 months starting from the date of receipt.
Target
Background	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids (By similarity).
Tissue specificity	Mainly expressed in epidermis.
Celluar localization	Endoplasmic reticulum;
UniPort	Q8TDN7