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Rabbit Anti-AWAT1 Polyclonal Antibody#abs138160

Please note that the price mentioned is only for your reference. For detailed pricing and information, kindly get in touch with our sales representative, Vecent. Western blot analysis AWAT1 using NIH-3T3 whole cell lysates This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs138160-100ug	1-2 Weeks	301.0
abs138160-50ug	1-2 Weeks	201.0

Please note that the price mentioned is only for your reference. For detailed pricing and information, kindly get in touch with our sales representative, Vecent.

Overview
catalog	abs138160
Other names	AWAT1, also known as Acyl-CoA wax alcohol acyltransferase 1, is an important enzyme involved in lipid metabolism. It plays a crucial role in the synthesis of wax esters and the accumulation of neutral lipids such as diacylglycerols. AWAT1 has also been referred to as AWAT1_HUMAN, DGA, DGA2, Diacyl glycerol acyltransferase 2, and Long-chain-alcohol O-fatty-acyltransferase 1. Additionally, there is another enzyme known as Diacylglycerol acyltransferase 2 (DGAT2), which shares some similarities with AWAT1. DGAT2, also called Diacylglycerol O acyltransferase 2 or Diacylglycerol O-acyltransferase 2-like protein 3, is involved in the synthesis of triglycerides from diacylglycerols. DGAT2-like protein 3 is another variant of this enzyme. These enzymes are crucial for lipid metabolism and have implications for various physiological processes, including energy storage and membrane structure. The rearrangement of the content above provides a concise overview of these enzymes and their functions.
Source	Rabbit
Specificity	The expression of total AWAT1 can be detected using the AWAT1 antibody, which specifically targets endogenous levels of this protein. To summarize, the antibody is designed to recognize and measure the overall amount of AWAT1 present in a biological sample.
Species Reactivity	Human;Rat
Antigen	AWAT1
Application	The recommended dilutions for western blotting are 1:1000 to 1:3000, for immunofluorescence/immunocytochemistry they are 1:100 to 1:500, and for peptide ELISA, the dilution ranges from 1:20000 to 1:40000. It is important to follow the recommended dilutions to achieve accurate and reliable results in these assays. Dilutions outside of these ranges may result in false positives or negatives, and can significantly impact the quality of your data. Therefore, it is crucial to optimize the appropriate dilution for your target protein or peptide to ensure the best possible results and minimize variability.
Immunogen	A synthesized peptide derived from human AWAT1.
MW	37 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification via peptide affinity chromatography. It was a rigorous process to ensure that the resulting product is of the highest quality possible. Through these methods, we were able to effectively isolate the desired components and create an antiserum that is well-suited for its intended purpose.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure proper storage, it is recommended to keep the item at a temperature of -20 °C for a period of one year. It is important to avoid repeatedly freezing and thawing the item as this can affect its quality.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C and has a stable shelf life of 12 months from the date of receipt. Please take care to keep this product at the recommended temperature and do not use past the expiration date.
Target
Background	Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has a preference for arachidyl alcohol as well as decyl alcohol, demonstrating its relatively poor activity using saturated long chain alcohols (C16, C18, and C20).
Tissue specificity	Predominantly expressed in skin, where it is limited to the sebaceous gland. Expressed in more mature, centrally located cells just before their rupture and sebum release. Also expressed in all tissues except spleen. Expressed at higher level in thymus, prostate and testis.
Celluar localization	Endoplasmic reticulum;
UniPort	Q58HT5