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Rabbit Anti-API-5 Polyclonal Antibody#abs133817

Please note that the price mentioned above is only for your reference. For the detailed price, we kindly request you to contact our seller, Vecent. Western blot analysis of extracts from 293 cells, Jurkat cells and HUVEC cells, using API-5 antibody. This product is for research use only, not for...

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Description

Catalog-specification	Delivery time	USD price
abs133817-100ug	1-2 Weeks	301.0
abs133817-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For the detailed price, we kindly request you to contact our seller, Vecent.

Overview
catalog	abs133817
Other names	The protein AAC 11, also known as Antiapoptosis clone 11 protein or API 5, plays a crucial role in inhibiting apoptosis. It is encoded by the API5 gene and is also referred to as API5l1. Another name for this protein is Cell migration inducing gene 8 protein (MIG 8) or Protein XAGL. Additionally, it is associated with Fibroblast growth factor 2 interacting factor 2 (FIF) or Fibroblast growth factor 2-interacting factor. The function of API 5 is to induce cell migration and inhibit apoptosis, making it a significant player in cellular processes.
Source	Rabbit
Specificity	The API-5 Antibody is capable of detecting the total endogenous presence of API-5. To create similar content based on this information, one could say: The total amount of API-5 within an organism can be detected using the API-5 Antibody, revealing endogenous levels of the protein.
Species Reactivity	Human;Mouse
Predictive reaction species	Horse
Antigen	API-5
Application	For Western blotting, a dilution of 1:500 to 1:1000 is recommended, while for immunohistochemistry, a dilution of 1:50 to 1:200 is suggested. When it comes to immunofluorescence or immunocytochemistry, a dilution of 1:100 to 1:500 is appropriate. For ELISA using peptide antigens, a dilution of 1:20000 to 1:40000 is optimal. These recommended dilutions will ensure the best results for your experiments.
Immunogen	A synthesized peptide.
MW	58 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Ensure that the product is stored at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze and thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Tissue specificity	Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.
Posttranslational modification	Acetylation at Lys-251 impairs antiapoptotic function.
Celluar localization	Nucleus;
UniPort	Q9BZZ5