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Rabbit Anti-APC8 Polyclonal Antibody#abs138157

Important: Please note that the price provided is only for your reference. For detailed pricing information, we recommend contacting our seller, Vecent. Western blot analysis APC8 using HuvEc whole cell lysates This product is for research use only, not for use in diagnostic prodecures or in...

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Description

Catalog-specification	Delivery time	USD price
abs138157-100ug	1-2 Weeks	301.0
abs138157-50ug	1-2 Weeks	201.0

Important: Please note that the price provided is only for your reference. For detailed pricing information, we recommend contacting our seller, Vecent.

Overview
catalog	abs138157
Other names	APC8, also known as Anaphase promoting complex subunit 8 or Cdc23, is a crucial component of the Anaphase-promoting complex. This protein plays an essential role in regulating the cell cycle by promoting the proteolysis of key cell cycle proteins, such as cyclin B and securin, during mitosis. Cell division cycle protein 23 homolog, also known as Cut23, is the human homolog of CDC23 and is involved in the same cellular processes. As part of the Cyclosome complex, APC8 controls the transition from metaphase to anaphase by targeting substrates for ubiquitination and degradation. Understanding the function of APC8 is crucial in investigating the mechanisms that regulate the cell cycle, and its dysregulation has been implicated in various cancers and developmental disorders.
Source	Rabbit
Specificity	The total APC8 levels in the body can be detected using the APC8 Antibody, which specifically targets the endogenous APC8 protein. ensuring reliable and accurate measurements.
Species Reactivity	Human;Mouse
Predictive reaction species	Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;
Antigen	APC8
Application	WB1:1000-3000，IF/ICC1:100-1:500，ELISA(peptide)1:20000-1:40000。，。
Immunogen	A synthesized peptide derived from human APC8.
MW	68 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography. To achieve this, a highly specific purification process was employed to ensure the removal of unwanted substances. This method resulted in a highly purified antiserum product with exceptional specificity and purity, making it ideal for further analysis and use in research applications.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to multiple cycles of freezing and thawing, as this can have a negative impact on its properties. Overall, proper storage conditions are essential to ensure optimal performance of the product.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4. The solution should also contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that the stability of this product is guaranteed for a period of 12 months from the date of receipt.
Target
Background	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Posttranslational modification	Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.
Celluar localization	Cytosol;Nucleus;
UniPort	Q9UJX2