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Phospho-Smad3 (Ser423+Ser425) Rabbit Polyclonal Antibody#abs140144

Phospho-Smad3 (Ser423+Ser425) Rabbit Polyclonal Antibody#abs140144

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Catalog-specificationDelivery timeUSD price

abs140144-100ug

In Stock

301.0

abs140144-50ug

In Stock

201.0

Please be informed that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to contact our seller, Vecent. Please note that the content generated by ChapGPT follows a different approach, so the conversation may have a completely different tone and structure.


Overview

catalog

abs140144
Other namesSMAD3, also known as hSMAD3 or mothers against decapentaplegic homolog 3, is a protein that is encoded by several different gene names, including DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD homolog 3, MAD homolog JV15 2, Mad protein homolog, MGC60396, Mothers against DPP homolog 3, SMA and MAD related protein 3, and SMAD family member 3.
SMAD3 is a key signaling molecule in the transforming growth factor-beta (TGF-β) signaling pathway and is involved in a variety of physiological processes, including cell proliferation and differentiation, apoptosis, and tissue homeostasis. It is activated by phosphorylation and translocates to the nucleus where it binds to DNA and regulates gene expression.
Disruption of the SMAD3 signaling pathway has been linked to several diseases, including cancer, fibrosis, and cardiovascular disorders. Therefore, understanding the function and regulation of SMAD3 is crucial for the development of new therapeutic strategies for these diseases.
SourceRabbit
SpecificityThe Smad3 (Phospho-Ser423+Ser425) Antibody is specialized in detecting the natural levels of Smad3 that possess phosphorylation at Ser423+Ser425. It is important to note that this antibody does not detect Smad3 that lacks the stated phosphorylation sites.
Species ReactivityHuman;Mouse
AntigenSmad2/Smad3
Application Western Blot（WB），1:1000-3000。（IF/ICC），1:100-1:500。（ELISA），1:20000-1:40000。。
ImmunogenOne possible rearranged content based on the original text information is:
A synthetic peptide has been developed that is derived from human Smad3 and phosphorylated at serine residues 423 and 425. This peptide sequence exhibits high similarity to the endogenous protein, allowing for accurate and specific targeting of Smad3 signaling pathways. The phosphorylation of these serine residues is known to regulate Smad3 activity, making this peptide a valuable tool for studying the role of Smad3 in various cellular processes. The peptide can also be used as a therapeutic agent for diseases that involve dysregulated Smad3 signaling, such as cancer and fibrosis. Overall, the development of this synthesized peptide provides a novel and effective approach for manipulating Smad3 activity and studying its physiological functions.
MW58kDa
Properties

Concentration

1mg/ml

purificationThe purified rabbit serum antibody was obtained through affinity purification using sequential chromatography on phospho- and non-phospho-peptide affinity columns.
ClonalityPolyclonal Antibody
Stability & StorageTo preserve the material for an extended period, it is recommended to keep it at -20 °C. Make sure to prevent any potential damage to the substance by avoiding repeated freeze and thaw cycles. This storage method is suitable for a year's duration.
Storage bufferRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Posttranslational modificationPhosphorylated on serine and threonine residues. Enhanced phosphorylation in the linker region on Thr-179, Ser-204 and Ser-208 on EGF and TGF-beta treatment. Ser-208 is the main site of MAPK-mediated phosphorylation. CDK-mediated phosphorylation occurs in a cell-cycle dependent manner and inhibits both the transcriptional activity and antiproliferative functions of SMAD3. This phosphorylation is inhibited by flavopiridol. Maximum phosphorylation at the G1/S junction. Also phosphorylated on serine residues in the C-terminal SXS motif by TGFBR1 and ACVR1. TGFBR1-mediated phosphorylation at these C-terminal sites is required for interaction with SMAD4, nuclear location and transactivational activity, and appears to be a prerequisite for the TGF-beta mediated phosphorylation in the linker region. Dephosphorylated in the C-terminal SXS motif by PPM1A. This dephosphorylation disrupts the interaction with SMAD4, promotes nuclear export and terminates TGF-beta-mediated signaling. Phosphorylation at Ser-418 by CSNK1G2/CK1 promotes ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Phosphorylated by PDPK1.Acetylation in the nucleus by EP300 in the MH2 domain regulates positively its transcriptional activity and is enhanced by TGF-beta.Poly-ADP-ribosylated by PARP1 and PARP2. ADP-ribosylation negatively regulates SMAD3 transcriptional responses during the course of TGF-beta signaling.Ubiquitinated. Monoubiquitinated, leading to prevent DNA-binding (PubMed:21947082). Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes (PubMed:21947082). Ubiquitinated by RNF111, leading to its degradation: only SMAD3 proteins that are 'in use' are targeted by RNF111, RNF111 playing a key role in activating SMAD3 and regulating its turnover (By similarity). Undergoes STUB1-mediated ubiquitination and degradation (PubMed:24613385).
Celluar localizationCytosol;Nucleus;Plasma Membrane;
UniPortP84022



Western blot analysis Smad3 (Phospho-Ser423+Ser425) using UV treated HeLa whole cell lysates


This product is for research use only, not for use in diagnostic prodecures or in human.


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