Phospho-NDR1/2 (Thr444/442) Rabbit Polyclonal Antibody#abs140003
Please note that the price provided is for your reference only. For detailed pricing information, we kindly ask you to contact our seller, Vecent. Western blot analysis NDR1/2 (Phospho-Thr444/442) using EGF treated HepG2 whole cell lysates This product is for research use only, not for use in...
Description
| Catalog-specification | Delivery time | USD price |
abs140003-100ug | 1-2 Weeks | 301.0 |
abs140003-50ug | 1-2 Weeks | 201.0 |
Please note that the price provided is for your reference only. For detailed pricing information, we kindly ask you to contact our seller, Vecent.
| Overview | |
catalog | abs140003 |
| Other names | NDR1 and NDR2 are two closely related serine/threonine protein kinases that belong to the NDR kinase family. NDR1 is also known as Nuclear Dbf2-related kinase 1, while NDR2 is also called Nuclear Dbf2-related kinase 2. Both kinases play important roles in regulating cell cycle progression, cell polarity, and apoptosis. NDR1 and NDR2 share high sequence similarity and identical substrate specificity. They are activated by the binding of their regulatory subunits MOB1A and MOB1B, which promote a conformational change to the kinase domain and enhance its catalytic activity. Once activated, NDR1 and NDR2 can phosphorylate a variety of downstream targets, such as LATS1/2, Cofilin, and YAP. Mutations in NDR1 and NDR2 genes have been associated with several human diseases, including cancer, neurological disorders, and developmental defects. Thus, both kinases are potential therapeutic targets for these diseases. In addition to NDR1 and NDR2, there is also a related protein kinase called STK38 that shares significant similarity with NDR1, but has distinct regulatory mechanisms and cellular functions. STK38 has been implicated in regulating actin cytoskeleton dynamics and stress response pathways. Overall, these NDR-related kinases are crucial regulators of various cellular processes and offer promising targets for disease intervention. |
| Source | Rabbit |
| Specificity | The NDR1/2 (Phospho-Thr444/442) Antibody specifically recognizes the phosphorylated form of NDR1/2 at Thr444/442. It is capable of detecting the endogenous levels of NDR1/2 when they are phosphorylated at this particular site. To obtain comparable information, I suggest rearranging the provided content while maintaining the context. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Xenopus;Rabbit;Pig;Dog;Sheep;Bovine;Horse |
| Antigen | NDR1/2 |
| Application | The recommended dilution for Western Blotting (WB) is 1:1000-3000, for Immunohistochemistry (IHC) is 1:50-1:200, and for ELISA (peptide) is 1:20000-1:40000. Please note that the generated content will vary significantly from the original text information. |
| Immunogen | NDR1/2（Phospho-Thr444/442）。 |
| MW | 54KD |
| Properties | |
Concentration | 1mg/ml |
| purification | The purified rabbit serum was used to generate the antibody, which was obtained through affinity purification using both phospho- and non-phospho-peptide affinity columns. This process involved sequential chromatography to isolate and purify the antibody. The resulting product is highly specific and can be used for a range of applications in biomedical research. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2. |
| Tissue specificity | Ubiquitously expressed with highest levels observed in peripheral blood leukocytes. |
| Posttranslational modification | ISGylated.Phosphorylated by STK3/MST2 and this is enhanced by MOBKL1B. |
| Celluar localization | Cytosol;Nucleus; |
| UniPort | Q15208/Q9Y2H1 |
Western blot analysis NDR1/2 (Phospho-Thr444/442) using EGF treated HepG2 whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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