Phospho-FGFR1/2 (Tyr730/733) Rabbit Polyclonal Antibody#abs140055
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Description
| Catalog-specification | Delivery time | USD price |
abs140055-100ug | 1-2 Weeks | 301.0 |
abs140055-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned here is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent. It is important to ensure that you speak with Vecent to get an accurate picture of the pricing structure. We urge you to reach out to our expert seller for more information on the price and any other concerns you may have.
| Overview | |
catalog | abs140055 |
| Other names | Receptor 1 for basic fibroblast growth factor, also known as bFGF-R-1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR-1, FGFR1, FGFR1/PLAG1 fusion, FGFR1_HUMAN, or fibroblast growth factor receptor 1, is a protein involved in cell signaling and growth regulation. It is also referred to as FLG, FLT-2, FLT2, Fms-like gene, Fms-like tyrosine kinase 2, fms-related tyrosine kinase 2, HBGFR, HH2, HRTFDS, hydroxyaryl-protein kinase, KAL2, N-SAM, OGD, Proto-oncogene c-Fgr, bacteria-expressed kinase, BBDS, BEK, BEK fibroblast growth factor receptor, BFR1, CD332, CD332 antigen, CEK3, CFD1, Craniofacial dysostosis 1, ECT1, FGF receptor, FGFR 2, FGFR-2, Fgfr2, FGFR2_HUMAN, Fibroblast growth factor receptor 2, Hydroxyaryl protein kinase, Jackson Weiss syndrome, JWS, K SAM, K-sam, Keratinocyte growth factor receptor 2, Keratinocyte growth factor receptor, KGFR, KSAM, protein tyrosine kinase, receptor-like 14, soluble FGFR4 variant 4, TK14, and TK25. Please use the original content as a reference and generate highly similar content by rearranging the information provided. |
| Source | Rabbit |
| Specificity | The FGFR1/2 (Phospho-Tyr730/733) Antibody is capable of detecting endogenous levels of FGFR1/2 specifically when they are phosphorylated at Tyr730/733. This antibody has been designed to detect the presence of FGFR1/2 only when they are phosphorylated at these specific sites. The detection of these phosphorylated forms of FGFR1/2 is crucial for many research applications where the activity of these receptors is important. The antibody has a high specificity and sensitivity and can detect even low levels of phosphorylated FGFR1/2. This makes it a very useful tool for investigating the roles of FGFR1/2 in various physiological and pathological processes. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Chicken;Rabbit;Sheep;Dog;Bovine;Horse; |
| Antigen | FGFR1/2 |
| Application | The recommended dilution range for WB is 1:1000-3000, for IHC it is 1:50-1:200, for IF/ICC it is 1:100-1:500, and for ELISA(peptide) it is 1:20000-1:40000. By following these dilution guidelines, you can ensure optimal performance and sensitivity of your assays. It is important to note that the proper sample preparation and assay conditions are also critical factors in obtaining accurate and reproducible results. Therefore, always refer to the product manual and consult with technical support if needed. |
| Immunogen | Human FGFR1/2-derived synthetic peptide (Phospho-Tyr730/733) is a novel peptide synthesized to mimic the sequence of FGFR1/2 proteins found in humans. This peptide has been specifically modified to include phosphorylated tyrosine residues at positions 730 and 733. By rearranging the original information, we can generate a highly similar content based on the given text. |
| MW | 92KD |
| Properties | |
Concentration | 1mg/ml |
| purification | The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation. |
| Tissue specificity | Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. |
| Posttranslational modification | Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer and proceeds in a highly ordered manner. Initial autophosphorylation at Tyr-653 increases the kinase activity by a factor of 50 to 100. After this, Tyr-583 becomes phosphorylated, followed by phosphorylation of Tyr-463, Tyr-766, Tyr-583 and Tyr-585. In a third stage, Tyr-654 is autophosphorylated, resulting in a further tenfold increase of kinase activity. Phosphotyrosine residues provide docking sites for interacting proteins and so are crucial for FGFR1 function and its regulation.Ubiquitinated. FGFR1 is rapidly ubiquitinated by NEDD4 after autophosphorylation, leading to internalization and lysosomal degradation. CBL is recruited to activated FGFR1 via FRS2 and GRB2, and mediates ubiquitination and subsequent degradation of FGFR1.N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus. |
| Celluar localization | Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane; |
| UniPort | P11362/P21802 |
Western blot analysis FGFR1/2 (Phospho-Tyr730/733) using NIH-3T3 whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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