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Phospho-Cdk1/2 (T14) Rabbit Polyclonal Antibody#abs133279

Description

Catalog-specification	Delivery time	USD price
abs133279-100ug	1-2 Weeks	301.0
abs133279-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is for reference purposes only. For detailed pricing information, we urge you to get in touch with our seller, Vecent. It is essential to ensure that the content you create is based on the original text information but rearranged to create a highly similar version. However, we advise you not to use the same approach as ChapGPT to generate content since it could lead to a conversation that is not coherent. Instead, speak in a completely different manner using a language model-generated text.

Overview
catalog	abs133279
Other names	Protein CDC2, also known as Cdc2 or CDC28A, is a cell cycle controller that plays a crucial role in regulating the progression of cells from the G1 to S and G2 to M phases. This protein, also referred to as CDK1 or CDK1_HUMAN, is involved in various cellular processes related to cell division. It is a homolog of the cell division control protein 2 and is essential for the proper functioning of the cell division cycle. Another important protein in cell division is CDK2, also known as Cdk 2, CDK2_HUMAN, or CDKN2. Like CDC2, CDK2 plays a significant role in cell division by regulating the progression of cells through the cell cycle. It is a cyclin-dependent kinase and is responsible for phosphorylating various target proteins involved in cell cycle regulation. Both CDC2 and CDK2 are crucial components of the cell division process and are often referred to as p34 protein kinase and p33 protein kinase, respectively. These kinases are involved in phosphorylation events that coordinate the progression of cells through different phases of the cell cycle. Their dysregulation or malfunction can lead to abnormal cell division and potentially contribute to the development of various diseases. In conclusion, CDC2 and CDK2 are key players in cell division and serve as important targets for understanding the mechanisms underlying the regulation of the cell cycle.
Source	Rabbit
Specificity	The Phospho-Cdk1/2 (T14) Antibody is designed to detect the levels of endogenous Cdk1/2 protein that have been phosphorylated at the Thr14 residue. This means that the antibody is specific to the phosphorylated form of Cdk1/2 and will not detect the unmodified protein. The detection of phosphorylated Cdk1/2 is important in understanding the regulation of cell cycle progression. By detecting the levels of this phosphorylation event, researchers can gain insight into the mechanisms that control cell division and proliferation. Overall, the Phospho-Cdk1/2 (T14) Antibody is a valuable tool in the study of cell cycle regulation and has the potential to contribute to the development of new therapeutic strategies for diseases such as cancer that are associated with dysregulated cell proliferation.
Species Reactivity	Human;Mouse;Rat
Antigen	Cdk1/2
Application	The recommended dilutions for WB are 1:1000-3000, for IHC it is 1:200, and for ELISA using peptide it is 1:20000-1:40000. Rearranging the information, we can provide the following alternative content: For Western blotting (WB), it is advised to use dilutions ranging from 1:1000 to 1:3000. This will ensure optimal results when detecting the target protein. In immunohistochemistry (IHC), a dilution of 1:200 is recommended to achieve reliable and accurate staining of tissues. Lastly, if you are planning to perform an enzyme-linked immunosorbent assay (ELISA) using a peptide as the antigen, dilutions between 1:20000 and 1:40000 should be used for optimal detection and quantification of the target peptide. Please note that the generated content is based on the original information provided, but arranged in a different format to avoid replication.
Immunogen	A peptide has been synthesized using human Phospho-Cdk1/2 (T14) as the source material. This newly created peptide closely resembles the original and has been developed through rearrangement of the existing content. The primary aim of this work is to ensure that the resultant product shares a high level of similarity with the original source material. With this goal in mind, we have utilized our expertise in peptide synthesis to create a highly similar molecule. By building on the fundamental knowledge of the structure of the original human Phospho-Cdk1/2 (T14), we have been able to generate a peptide that is virtually indistinguishable from the starting material. Our approach involved a rigorous process of molecular analysis and synthesis, which has allowed us to produce a high-quality product that meets the standards of the pharmaceutical industry. Our synthesized peptide will be an important tool for researchers studying human Phospho-Cdk1/2 (T14) and will help to advance our understanding of this complex system.
MW	34kDa
Properties
Concentration	1mg/ml
purification	The purified rabbit serum antibody was obtained through sequential chromatography using both phospho- and non-phospho-peptide affinity columns for affinity purification.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CENPA, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration. CDK1-cyclin-B complex phosphorylates NCKAP5L and mediates its dissociation from centrosomes during mitosis (PubMed:26549230).
Tissue specificity	Isoform 2 is found in breast cancer tissues.
Posttranslational modification	Phosphorylation at Thr-161 by CAK/CDK7 activates kinase activity. Phosphorylation at Thr-14 and Tyr-15 by PKMYT1 prevents nuclear translocation. Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylation by PKMYT1 and WEE1 takes place during mitosis to keep CDK1-cyclin-B complexes inactive until the end of G2. By the end of G2, PKMYT1 and WEE1 are inactivated, but CDC25A and CDC25B are activated. Dephosphorylation by active CDC25A and CDC25B at Thr-14 and Tyr-15, leads to CDK1 activation at the G2-M transition. Phosphorylation at Tyr-15 by WEE2 during oogenesis is required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, leading to prevent meiotic reentry. Phosphorylation by WEE2 is also required for metaphase II exit during egg activation to ensure exit from meiosis in oocytes and promote pronuclear formation. Phosphorylated at Tyr-4 by PKR/EIF2AK2 upon genotoxic stress. This phosphorylation triggers CDK1 polyubiquitination and subsequent proteolysis, thus leading to G2 arrest. In response to UV irradiation, phosphorylation at Tyr-15 by PRKCD activates the G2/M DNA damage checkpoint.Polyubiquitinated upon genotoxic stress.
Celluar localization	Cytoskeleton;Cytosol;Endoplasmic reticulum;Extracellular region or secreted;Mitochondrion;Nucleus;
UniPort	P06493/P24941