Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Phospho-CdGAP (Thr789) Rabbit Polyclonal Antibody#abs140081

Please note that the price provided is for your reference only. If you require detailed pricing information, please contact our seller Vecent. Kindly be advised that the content generated by ChapGPT should not be used as a basis for conversation. Instead, we request that you generate text using...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs140081-100ug	1-2 Weeks	301.0
abs140081-50ug	1-2 Weeks	201.0

Overview
catalog	abs140081
Other names	The Rho GTPase-activating protein 31, also known as ARHGAP31 or CDC42 GTPase-activating protein, is an important molecule involved in cellular processes. It plays a crucial role in the regulation of the Cdc42 GTPase protein, which is responsible for controlling cell migration, polarity, and cytoskeletal organization. The ARHGAP31 protein, encoded by the RHG31_HUMAN gene, acts as a GTPase-activating protein (GAP) for the Cdc42 protein. Its main function is to accelerate the hydrolysis of GTP (guanosine triphosphate) to GDP (guanosine diphosphate) on the Cdc42 protein. This GTP hydrolysis leads to the inactivation of Cdc42 and subsequent downstream signaling events. ARHGAP31 is widely expressed in various tissues, including the brain, heart, and skeletal muscle. It is involved in various cellular processes, such as cell adhesion, migration, and proliferation. Dysregulation of ARHGAP31 has been associated with several diseases, including cancer metastasis, neuronal disorders, and cardiovascular diseases. Understanding the molecular mechanisms underlying the function of ARHGAP31 is essential for elucidating its role in normal cellular processes and disease pathogenesis. Further studies are needed to uncover the specific interactions and regulatory networks of ARHGAP31 in different cellular contexts. In conclusion, the protein ARHGAP31, also known as CDC42 GTPase-activating protein, is a crucial player in cellular processes by regulating the activity of Cdc42. Its dysregulation has implications in various diseases, highlighting the importance of further research in this field.
Source	Rabbit
Specificity	The CdGAP (Phospho-Thr789) Antibody specifically recognizes the phosphorylation of CdGAP at Thr789. It efficiently detects the naturally occurring levels of phosphorylated CdGAP protein. By targeting this specific site of phosphorylation, the antibody provides valuable insight into the cellular mechanisms and signaling pathways involving CdGAP.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Sheep;Dog;Bovine;
Antigen	CdGAP
Application	The recommended dilutions for WB are 1:1000-3000, for IHC they are 1:50-1:200, and for ELISA (peptide) they are 1:20000-1:40000. It is important to adhere to these dilutions in order to obtain reliable and accurate results. For WB, it is recommended to use the higher dilutions for stronger signals while for IHC, the lower dilutions may be more appropriate for better visualization of staining. ELISA dilutions may need to be optimized based on the specific peptide being used. Overall, careful attention to dilution factors is critical for obtaining reproducible data in these assays.
Immunogen	Human CdGAP (Phospho-Thr789) is the source of a synthesized peptide that has been derived. It is important to note that the generated content will be highly similar to the original text given the restrictions imposed, but it will be presented in a completely different manner using language model-generated text.
MW	157KD
Properties
Concentration	1mg/ml
purification	The affinity-purified antibody was obtained from rabbit serum and underwent sequential chromatography on both phospho- and non-phospho-peptide affinity columns to ensure its high quality. This process guarantees a highly specific and effective antibody that can accurately detect the target protein.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to keep it stored at a temperature of -20 °C for a period of one year. It is important to avoid any fluctuations in temperature and continuous freeze/thaw cycles. Therefore, proper handling and storage techniques should be followed to ensure the longevity of the product.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it is suspended in phosphate buffered saline at pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C. The stability of Rabbit IgG is ensured for a period of 12 months from the date of receipt.
Target
Background	Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration.
Tissue specificity	Mainly expressed in fetal heart and muscle.
Posttranslational modification	Phosphorylation on Thr-789 reduces GAP activity.
Celluar localization	Cytosol;
UniPort	Q2M1Z3