Phospho-Catenin- Beta (Ser33) Rabbit Polyclonal Antibody#abs130894

abs130894-50ug

1-2 Weeks

201

abs130894-100ug

1-2 Weeks

301

Description

Adherens junctions, also known as zonula adherens, play a crucial role in maintaining epithelial layers, which line organ surfaces. Beta-catenin, an important adherens junction protein, facilitates adhesion between cells, informs neighboring cells of their presence, and anchors the actin cytoskeleton. As a result, AJs regulate normal cell growth and behavior. A better understanding of the functions of beta-catenin in AJs may pave the way for the development of therapies for diseases characterized by disruptions in AJs and their associated pathways.

Other names

Beta-catenin, also known as catenin (cadherin associated protein), is an essential component of the Wnt signaling pathway. This protein plays a critical role in mediating cell adhesion and cell signaling through its interaction with cadherin, a protein that promotes cell-to-cell adhesion. Beta-catenin is a 88kDa protein and is a member of the catenin family. It is also known as Catenin beta 1, CTNNB1, and CHBCAT. In addition, this protein is encoded by the CTNNB1 gene, which is located on chromosome 3 in humans. Mutations in this gene have been implicated in various cancers, including colorectal cancer and hepatocellular carcinoma. Beta-catenin is a multifunctional protein that regulates gene expression, cell proliferation, differentiation, and apoptosis. Thus, understanding the mechanism of beta-catenin function has important implications for the development of targeted cancer therapies.

Source

Rabbit

Specificity

The Antibody for Phospho-Catenin-beta (Ser33) works by detecting Catenin-beta at its endogenous levels, but only when it undergoes phosphorylation at Serine 33. This means that the antibody is most effective in identifying the specific form of Catenin-beta that has undergone this modification. By using this antibody, researchers can better understand the role that phosphorylation of Serine 33 plays in the physiological and pathological functions of Catenin-beta.

Predictive reaction species

Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig

Application

The recommended dilution for using this antibody varies depending on the application. For Western blotting, a dilution range of 1:500 to 1:2000 is suggested. For immunohistochemistry, a dilution range of 1:50 to 1:200 is recommended. For immunofluorescence and immunocytochemistry, a dilution range of 1:100 to 1:500 is recommended. Finally, if using ELISA with peptide, a dilution range of 1:20000 to 1:40000 is suggested. It is important to note that dilution ranges may vary slightly based on individual experiments and sample type.

A peptide that has been synthesized from the human protein Catenin-beta and is based on the phosphorylation site of Serine 33 is available. This peptide is very similar to the original protein sequence and can be used in a variety of research applications. The unique properties of this peptide make it an ideal tool for studying the function and regulation of Catenin-beta in different biological contexts. By using this peptide, researchers can gain valuable insights into the molecular mechanisms that underlie the complex processes associated with this critical protein, and ultimately, advance our understanding of human biology and disease.

Properties

1mg/ml

Purification

The purification of the antibody involved sequential chromatography on affinity columns, specifically targeting both phospho- and non-phospho-peptides, derived from purified rabbit serum. To ensure a highly specific and purified antibody, this affinity purification method was employed.

Clonality

Polyclonal Antibody

To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this may affect its quality and stability.

Storage buffer

：-20°C（pH 7.4），150NaCl，0.02%（sodium azide）50%。12。。

Target

Background

 Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity).

Tissue specificity

Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level).

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;

P35222