Phospho-beta-Catenin (Ser33/37/Thr41) Rabbit Polyclonal Antibody#abs138008
Please note that the price provided above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Please refrain from using the chatGPT content generation approach and instead generate text using a completely different method from the language...
Description
| Catalog-specification | Delivery time | USD price |
abs138008-100ug | 1-2 Weeks | 301.0 |
abs138008-50ug | 1-2 Weeks | 201.0 |
Please note that the price provided above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Please refrain from using the chatGPT content generation approach and instead generate text using a completely different method from the language model.
| Overview | |
catalog | abs138008 |
| Other names | Beta-catenin, also known as cadherin-associated protein or catenin beta-1, is an 88kDa protein that plays an important role in cell adhesion and signaling pathways. It is often associated with cadherin proteins and facilitates cell-cell adhesion by connecting the cadherins to the actin cytoskeleton. Beta-catenin is also involved in the Wnt signaling pathway, which plays a crucial role in embryonic development and tissue homeostasis. Dysregulation of beta-catenin has been linked to various diseases, including cancer and developmental disorders. In humans, beta-catenin is encoded by the CTNNB1 gene and has several aliases, including CATNB, CHBCAT, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000162082, OTTHUMP00000165222, OTTHUMP00000165223, OTTHUMP00000209288, and OTTHUMP00000209289. |
| Source | Rabbit |
| Specificity | The Phospho-β-Catenin (Ser33/37/Thr41) Antibody is specifically designed to identify and measure the levels of β-Catenin protein that has undergone phosphorylation at Ser33/37/Thr41. It can accurately detect this modified form of β-Catenin in samples derived from biological systems. The antibody does not recognize or respond to the unphosphorylated β-Catenin protein. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | β-Catenin |
| Application | To achieve optimal results, we recommend using WB at dilution ratios of 1:1000-3000, and IF/ICC at dilution ratios of 1:100-1:500. When performing ELISA assays with peptides, we suggest using dilution ratios of 1:20000-1:40000. These dilution ratios have been proven effective and reliable in a variety of experimental conditions. |
| Immunogen | A peptide has been synthesized from human Phospho-β-Catenin (Ser33/37/Thr41), which shares a high level of similarity to its original sequence. The peptide is derived from these specific phosphorylated sites and is designed to mimic the protein's activity in biological pathways. This synthesized peptide offers a valuable tool for exploring the functions of Phospho-β-Catenin in cellular mechanisms and potential therapeutic applications. |
| MW | 92KD |
| Properties | |
Concentration | 1mg/ml |
| purification | The purified rabbit serum was used to obtain the antibody through affinity purification techniques involving sequential chromatography on phospho- and non-phospho-peptide affinity columns. The resulting antibody is highly specific and effective for its intended applications. Overall, this method ensures the production of high-quality antibodies that are suitable for various research purposes. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | To ensure optimal preservation, it is recommended to store at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles as this can have negative effects on its quality. |
| Storage buffer | This highly similar and rearranged content is as follows: The Rabbit IgG is suspended in phosphate buffered saline with a pH of 7.4. It contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C for optimal stability. This product remains stable for 12 months from the date of receipt. |
Target | |
Background | Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). |
| Tissue specificity | Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). |
| Posttranslational modification | Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription (By similarity). Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation (PubMed:25169422). Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity.Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation (By similarity).S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions.O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1.Deacetylated at Lys-49 by SIRT1. |
| Celluar localization | Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane; |
| UniPort | P35222 |
Western blot analysis Phospho-β-Catenin (Ser33/37/Thr41) using HepG2 whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
Hot Tags: phospho-beta-catenin (ser33/37/thr41) rabbit polyclonal antibody#abs138008, China phospho-beta-catenin (ser33/37/thr41) rabbit polyclonal antibody#abs138008 suppliers
Send Inquiry
You Might Also Like
