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Rabbit Anti-NR0B1 Polyclonal Antibody#abs136105

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Description

Catalog-specification	Delivery time	USD price
abs136105-100ug	1-2 Weeks	301.0
abs136105-50ug	1-2 Weeks	201.0

Overview
catalog	abs136105
Description	The protein encoded by this gene possesses a DNA-binding domain and serves as a vital regulator of transcription. It exerts its influence as a dominant-negative factor on retinoic acid receptor-mediated transcription. Additionally, this protein acts as an inhibitor for Sry, functioning as an anti-testis gene. When mutations occur in this particular gene, it leads to X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. This information was sourced from RefSeq in July 2008. Let me know if you need any further assistance.
Other names	The Nuclear hormone receptor DAX-1, also known as Dosage sensitive sex reversal (DSS) and DSS AHC critical region on the X chromosome protein 1, is a critical protein involved in sex development and steroidogenesis. It is encoded by the NR0B1 gene, also called NROB1 or Nuclear receptor subfamily 0 group B member 1. Mutations in the NR0B1 gene can lead to various disorders, including adrenal hypoplasia congenita (AHC), hypogonadotropic hypogonadism (HHG), and glycerol kinase deficiency (GTD). These conditions affect the development of the adrenal gland and gonads, leading to inadequate production of hormones. DAX-1 is a transcriptional repressor that regulates the expression of several genes involved in hormone synthesis and sexual differentiation. It interacts with other nuclear receptors, such as steroidogenic factor 1 (SF-1) and liver receptor homolog 1 (LRH-1), to modulate their activity. Research on DAX-1 has provided insights into the molecular mechanisms underlying sex determination and gonadal differentiation. Understanding its role may help in the development of treatments for disorders related to steroidogenesis and sex development.
Source	Rabbit
Specificity	The NR0B1 Antibody is capable of detecting total NR0B1 at its natural levels within the body. This indicates that the antibody is highly specific to NR0B1, enabling it to identify and quantify the protein with great accuracy.
Species Reactivity	Human;Mouse;Rat
Antigen	NR0B1
Application	The dilutions for Western blot (WB) range from 1:500 to 1:2000, for immunohistochemistry (IHC) range from 1:50 to 1:200, for immunofluorescence/immunocytochemistry (IF/ICC) range from 1:100 to 1:500, and for ELISA (peptide) range from 1:20000 to 1:40000. These dilution ranges are commonly used in the laboratory to achieve optimal staining and detection of specific proteins or peptides. It is important to carefully choose the appropriate dilution factor based on the specific experimental conditions and target molecule, ensuring reliable and accurate results.
Immunogen	A synthesized peptide derived from human NR0B1.
MW	52kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).
Celluar localization	Nucleus;
UniPort	P51843