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Rabbit Anti-SPAM1 Polyclonal Antibody#abs136364

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Description

Catalog-specification	Delivery time	USD price
abs136364-100ug	1-2 Weeks	301.0
abs136364-50ug	1-2 Weeks	201.0

Overview
catalog	abs136364
Description	Hyaluronidase is responsible for breaking down hyaluronic acid, a significant structural component of extracellular matrices and basement membranes. There are six members of the hyaluronidase family divided into two closely linked groups on chromosome 3p21.3 and 7q31.3. The official symbol for this gene is SPAM1, previously known as HYAL1 and HYA1. Another family member located on chromosome 3p21.3 is designated HYAL1. This gene codes for a GPI-anchored enzyme situated on the inner acrosomal membrane and surface of human sperm. This multifaceted protein acts as a hyaluronidase, which facilitates the penetration of sperm through the hyaluronic acid-rich cumulus cell layer around the oocyte. Additionally, it acts as a receptor that plays a part in cell signaling induced by hyaluronic acid and is involved in the adhesion of sperm to the zona pellucida. Its abnormal expression in tumors is linked to the degradation of basement membranes, culminating in tumor invasion and metastasis. Many transcript variants encoding various isoforms of this gene have been identified. [RefSeq, March 2010].
Other names	The protein known as epididymis secretory sperm binding protein Li 96n is also referred to as HEL-S-96n, HYA1, HYAL PH-20, HYAL1, HYAL3, HYAL5, HYALP_HUMAN, PH-20 Hyaluronidase, SPAG15, or Spam1. It plays a crucial role in sperm adhesion and is particularly involved in binding to the zona pellucida. This binding is facilitated by the hyaluronidase activity of this protein. Other names for this protein include Hyaluronoglucosaminidase PH-20, MGC26532, PH20 Hyaluronidase, SPAM-1, Sperm adhesion molecule 1, Sperm surface protein PH-20, and Sperm surface protein PH20. Please note that the rearranged content may not preserve all the semantic information, but it should still capture the essence of the original text.
Source	Rabbit
Specificity	The total SPAM1 protein can be accurately detected using the SPAM1 Antibody, which is capable of detecting the endogenous levels of this protein.
Species Reactivity	Human;Mouse;Rat
Antigen	SPAM1
Application	，： ELISA(peptide) 1:20000-1:40000，WB 1:500-1:2000，IHC 1:50-1:200。
Immunogen	A synthesized peptide derived from human SPAM1.
MW	58kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Tissue specificity	Testis.
Posttranslational modification	N-glycosylated.
Celluar localization	Plasma Membrane;
UniPort	P38567