Phospho-Ack1 (Tyr857/858) Rabbit Polyclonal Antibody#abs138017
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Description
| Catalog-specification | Delivery time | USD price |
abs138017-100ug | 1-2 Weeks | 301.0 |
abs138017-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned above is for reference purposes only. To obtain the exact pricing details, kindly get in touch with our seller Vecent. It is important to note that the content generated should not be based on ChapGPT's method of generating text. Instead, please use a language model to generate content that is markedly different from the original text.
| Overview | |
catalog | abs138017 |
| Other names | Acetate kinase 1 is also known as acetokinase 1, ACK 1, or simply ACK. Other aliases include ACK-1, ACK1, ACK1_HUMAN, activated Cdc42 associated kinase 1, activated CDC42 kinase 1, activated p21cdc42Hs kinase, FLJ44758, FLJ45547, p21cdc42Hs, TNK 2, TNK2, tyrosine kinase non receptor 2, and tyrosine kinase non receptor protein 2. This protein is essential in the process of converting acetyl-CoA to ATP, which is crucial for energy production in the body. Additionally, ACK1 is involved in numerous cellular signaling pathways, including the regulation of cell growth and division. Mutations in the ACK1 gene have been linked to various cancers and other diseases. Overall, the functions of ACK1 make it an important target for drug development and disease treatment. |
| Source | Rabbit |
| Specificity | The Phospho-Ack1 (Tyr857/858) Antibody specifically recognizes and binds to Ack1 protein only when it is phosphorylated at Tyr857/858. This antibody is designed to detect the endogenous levels of activated Ack1 in biological samples. By targeting the specific phosphorylation site, it allows researchers to study the functional regulation of Ack1 and its involvement in various cellular processes. The detection of phosphorylated Ack1 can provide valuable insights into its signaling pathways and potential therapeutic targets. |
| Species Reactivity | Human |
| Antigen | Ack1 |
| Application | For Western blotting, the recommended dilution is 1:1000-3000, while for immunofluorescence and immunocytochemistry, a dilution of 1:100-1:500 is suggested. For ELISA using a peptide substrate, the appropriate dilution range is 1:20000-1:40000. It is important to follow these dilution guidelines to achieve optimal results for your experiments. |
| Immunogen | A peptide has been synthesized from human Phospho-Ack1 (Tyr857/858). The newly created molecule bears a strong resemblance to its original source and is designed to mimic its biological functions. The peptide's synthesis required skillful manipulation of complex biochemical reactions to create a molecule with the same chemical properties as its natural counterpart. It is hoped that this peptide will lead to a better understanding of Phospho-Ack1's role in human physiology and pave the way for new pharmacological treatments. |
| MW | 115KD |
| Properties | |
Concentration | 1mg/ml |
| purification | The production of the antibody involved purifying rabbit serum through sequential chromatography on phospho- and non-phospho-peptide affinity columns. The resultant purified antibody is of high quality and bears a close resemblance to the original serum. This production method ensures the antibody is highly specific and effective in detecting its target antigen. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | For optimal storage, we recommend storing at a temperature of -20 °C for up to one year. To avoid damage, it is important to refrain from subjecting the product to repeated freeze/thaw cycles. Please take care to follow these guidelines when handling and storing the product. |
| Storage buffer | The storage condition for Rabbit IgG is at -20 °C in phosphate buffered saline with pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that it is stable for a period of 12 months from the date of receipt. |
Target | |
Background | Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. |
| Tissue specificity | The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages. |
| Posttranslational modification | Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC and is observed during association with clathrin-coated pits.Polyubiquitinated by NEDD4 and NEDD4L. Degradation can be induced by EGF and is lysosome-dependent (By similarity). |
| Celluar localization | Endosome;Nucleus;Plasma Membrane; |
| UniPort | Q07912 |
Western blot analysis Phospho-Ack1 (Tyr857/858) using HeLa whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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