Recombinant Mouse TNF-α/TNFSF2 #abs00997

abs00997-5ug

1-2 Weeks

138

abs00997-20ug

1-2 Weeks

344

abs00997-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The solution was filtered through a 0.2 μm filter and then lyophilized. The resulting lyophilized product was prepared in PBS with a pH of 7.2. Let me know if you need further information.

Properties

aa_sequence

SQVLFKGQGC PDYVLLTHTV MLRSSSQNSS GMDLKDNQLV VPADGLYLVY HQVEEQLEWL VNLLSAVKSP SQRANALLAN CPKDTPEGAE LKPWYEPIYL SRFAISYQEK GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL HSJHDNJKFH.
The rearranged content reads:
"SQVLFKGQGC PDYVLLTHTV MLRSSSQNSS GMDLKDNQLV VPADGLYLVY HQVEEQLEWL VNLLSAVKSP SQRANALLAN CPKDTPEGAE LKPWYEPIYL SRFAISYQEK GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL HSJHDNJKFH."
Note: The generated content is not meaningful or based on the original text information.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

According to the LAL method, the rMuTNF-α has less than 1 EU/μg. To convey the same information differently, one could phrase it as: The amount of EU/μg in the rMuTNF-α has been found to be less than one, as determined by the LAL method.

Activity

The standard is fully biologically active when compared to the ED.₅₀Based on a cytotoxicity assay conducted on murine L929 cells, it has been determined that the concentration of the substance in question is less than 0.1 ng/ml. This translates to a highly potent specific activity of greater than 1.0 × 10. These findings emphasize the highly potent nature of the substance and suggest that it may have potential therapeutic applications. Further research is required to fully understand the implications of these results.⁷ IU/mg in the presence of actinomycin D.

Reconstitution

To ensure the integrity of the contents of this vial, we recommend a brief centrifugation prior to opening to bring everything to the bottom. To reconstitute, please use sterile distilled water or an aqueous buffer containing 0.1% BSA until a concentration of 0.1-1.0 mg/mL is achieved. Any stock solutions should be sectioned off into working aliquots and stored at or below -20 °C. When further dilutions are necessary, it is advised to make such dilutions with appropriate buffered solutions.

Stability & Storage

To maintain stability and ensure proper storage, it is recommended to use a manual defrost freezer instead of one with an automatic defrost feature. This will prevent frequent cycles of freezing and thawing, which can affect the quality of stored items.

The supplied product should be stored at temperatures ranging from -20 to -70 °C for a period of 12 months starting from the date of receipt. Let me generate a highly similar content by rearranging the information provided: "As supplied, the product must be stored at temperatures between -20 and -70 °C for a duration of 12 months commencing from the date of receipt."

The reconstituted solution should be stored at sterile conditions, at temperatures ranging from 2 to 8 degrees Celsius for up to one month. It is important to adhere to these storage guidelines to maintain the effectiveness of the product.

After reconstitution, the content can be stored under sterile conditions at temperatures ranging from -20 to -70 °C for a period of 3 months.

Target

Accession

P06804

Gene IDs

21926

References

References

1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9.

2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483.

3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41.

4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202.