Recombinant Human SRANK Receptor/TNFRSF11A #abs00869

abs00869-20ug

1-2 Weeks

138

abs00869-100ug

1-2 Weeks

344

abs00869-1mg

1-2 Weeks

1425

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM NaCl was filtered through a 0.2 μm membrane and then lyophilized. To generate a similar content, the information can be rearranged to say that a highly concentrated solution was filtered through a 0.2 μm membrane, after which it was lyophilized. The original solution was in Tris-HCl buffer with a pH of 8.0 and a concentration of 150mM NaCl.

Properties

aa_sequence

CATPTKNSIQS CGCSLGRHNY EPNMGSSKCK CSTSVDTKCT LPGCYPDELH SEDKNLCWNE LCKGTAVCDH VNTSTAVVGA CYTACTRPCH SQDCECRCNW PAPGACLQET HANKDLQNLP CKPCLAGVYF KSTSDSFADR CRWTNCFPTL LVKREEHGTH KCSADCSSLA RAPSL.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method detected Recombinant Human RANK Receptor at a level of less than 0.1 EU/μg. To generate similar content, the information could be rearranged to say: The level of Recombinant Human RANK Receptor was determined using the LAL method and was found to be less than 0.1 EU/μg.

Activity

The potency of the test sample matches that of the standard in terms of biological activity. This means that the test sample is just as effective as the standard when it comes to carrying out its intended biological function.₅₀The specific activity of the substance can be determined by its effectiveness in inhibiting sRANK Ligand induced nuclear factor kappa B (NFκB) in RAW 264.7 cells. This specific activity is considered to be less than 50 ng/ml. Moreover, this activity is measured in terms of its capability to surpass a specific threshold of > 2.0 × 10.⁴In the presence of 15 ng/ml of recombinant sRANK Ligand, the activity of ALP was measured to be 12.5 IU/mg. To be more precise, the specific activity of ALP was determined under these conditions. This information is important for understanding the enzymatic activity of ALP in the presence of sRANK Ligand. Researchers can use this data to further explore the potential role of sRANK Ligand in regulating ALP activity. Overall, this data provides valuable insights into the biochemical mechanisms involved in bone formation and could have implications for the development of new treatments for bone disorders.

Reconstitution

To ensure proper mixing of the contents, it is recommended to briefly centrifuge the vial before opening. In order to reconstitute, utilize sterile distilled water or a buffered solution containing 0.1 % BSA to achieve a concentration of 0.1-1.0 mg/mL. For preservation, it is advisable to divide the solution into smaller working aliquots and store them at or below -20 °C. For further dilutions, utilize suitable buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to store it at 2-8°C. However, for long-term storage, it is highly recommended to keep it at -20°C, and preferably desiccated. After reconstitution, the preparation should be used within one week and stored at 2-8°C. To prolong its stability, it is best to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20°C to -70°C. It is crucial to avoid any repeated freeze-thaw cycles to maintain the preparation's quality.

Target

Accession

Q9Y6Q6

Gene IDs

8792