Recombinant Human Ubiquitin-Conjugating Enzyme E2 D1/UBE2D1/UbcH5a (N-GST) #abs04433

abs04433-10ug

1-2 Weeks

39

abs04433-50ug

1-2 Weeks

130

abs04433-500ug

1-2 Weeks

535

abs04433-1mg

1-2 Weeks

728

Description

Our E.coli expression system produces Recombinant Human Ubiquitin-conjugating enzyme E2 D1. The target gene encoding Met1-Met147 is expressed with a GST tag at the N-terminus.

Other names

D1 Ubiquitin-conjugating enzyme (UBE2D1), known as Stimulator of Fe transport (SFT), is a homolog of UBC4/5 and UbcH5. It is also referred to as Ubiquitin carrier protein D1 and Ubiquitin-protein ligase D1. Additionally, it is classified as Ubiquitin-conjugating enzyme E2 (17) KB 1 and Ubiquitin-conjugating enzyme E2-17 kDa 1. Another name associated with this enzyme is UBC5A, commonly known as UBCH5 or UBCH5A.

Source

Escherichia coli.

Format

The solution was filtered through a 0.2 μm filter and then lyophilized. The original solution consisted of 50mM HEPES, 150mM NaCl, 2mM DTT, and 10% glycerin with a pH of 7.5. The resulting content should closely match the original content, as it is based on the same information.

Properties

aa_sequence

LTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKS SKYIAWPLQGWQATFGGGDHPPKSDLVPRGSMALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAMMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHK.

Concentration

SDS-PAGE，95%。，，。

Endotoxin_level

The LAL test determined that the content is based on original text information, with a value of less than 0.1 ng/μg (1 IEU/μg). Let me generate highly similar content by rearranging the provided information.

Reconstitution

To ensure proper handling and optimal results, it is important to follow certain guidelines when working with lyophilized protein. Before opening the tube, always remember to centrifuge it first, and avoid mixing the contents by vortex or pipetting. When reconstituting the protein, it is generally not recommended to dilute it to a concentration less than 100 μg/ml. The lyophilized protein should be dissolved in ddH2O, and it is advisable to aliquot the reconstituted solution to minimize the number of freeze-thaw cycles. By adhering to these best practices, you can rest assured that your protein work will yield the most accurate and reliable results.

Target

Background

Ubiquitin-conjugating enzyme E2 D1 (UBE2D1) belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Accession

P51668