Recombinant Human TAFA-2 #abs01148

abs01148-5ug

1-2 Weeks

138

abs01148-20ug

1-2 Weeks

344

abs01148-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 2 × PBS, pH 7.4, was filtered through a 0.2 μm filter and subjected to lyophilization. The resultant product is a powder that retains the properties of the original solution.

Properties

aa_sequence

VTHCAETCGT SNRCKQCCKT HEKTVRAQCC VEPSWCISPQ GKRWECVDAS AHHQKMQWCE RVTTSVKGNK SVGAVPQCRE LIVSCCEKGW TRTANAQKHK. A similar content can be generated by reorganizing the above information as follows: "The sequence ANHHKAHHVK TGTCEVVALH RCCNKNKIEE RSQTVKCSCF PGQVAGTTRA APSCVDASIV EQKWWCHMQP CLEGEECKVL PDRKGWSCSS GNKVKTTRVT H can be rearranged to form a new text that reads: "VTHCAETCGT SNRCKQCCKT HEKTVRAQCC VEPSWCISPQ GKRWECVDAS AHHQKMQWCE RVTTSVKGNK SVGAVPQCRE LIVSCCEKGW TRTANAQKHK." Despite the different order of the sequences, the overall information in the original text remains unchanged in the new generated content.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the Recombinant Human TAFA-2 contains less than 1 EU/μg.

Activity

Compared to the standard, the recombinant Human TAFA-2 exhibits full biological activity. Its effectiveness is measured by its ability to boost neurite outgrowth of E16-E18 rat embryonic cortical neurons. When immobilized at concentrations ranging from 6-24 μg/mL on a 96 well plate, it has demonstrated a significant improvement in neurite outgrowth. Essentially, this indicates that the recombinant Human TAFA-2 is an excellent option for enhancing neuronal growth and development.

Reconstitution

To ensure the contents settle at the bottom, we advise gently centrifuging this vial before opening. To prepare, add sterile distilled water or an aqueous buffer containing 0.1% BSA until the concentration reaches 0.1-1.0 mg/mL. It is recommended to divide stock solutions into working aliquots and store them at temperatures not exceeding -20 °C. For further dilutions, use appropriate buffered solutions.

Stability & Storage

For optimal storage of this lyophilized preparation, it should be kept at -20 °C, preferably desiccated. However, it can also be stored at 2-8 °C without compromising its stability. When reconstituted, the preparation remains stable for one week at 2-8 °C. To maximize its stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles as this may affect its quality.

Target

Accession

Q8N3H0

Gene IDs

338811

References

References

1. Tom Tang Y, Emtage P, Funk WD, et al. 2004. Genomics, 83: 727-34.

2. Sambo MH, Lewis I, Idris SH. 2010. Niger J Med, 19: 194-8.

3. Fischer C, Christ E, Korf HW, et al. 2012. Gen Comp Endocrinol, 177: 98-103.