Recombinant Human PTN #abs00932

abs00932-5ug

1-2 Weeks

138

abs00932-20ug

1-2 Weeks

344

abs00932-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The solution was filtered through a 0.2 μm filter and then lyophilized. The resulting product was reconstituted with PBS buffer and adjusted to a pH of 7.4. This method ensured that the final product was pure and free of contaminants.

Properties

aa_sequence

LKMPQKQKQ CGKCLSDWVE GECGLSDPTV GREGTTGTRE CQMKTQCKEK NRPCKIKCQF GAEQYKFQWC ECDNGWALFQ STGRTKTLAK QEHANRLAKR KPCSITVTKV PKQPKKETKA EKGSKKKEKK DLMKQEKEQ

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the Recombinant Human PTN contains less than 0.1 EU/μg. A highly similar content can be generated by rearranging the information as follows: The Recombinant Human PTN was tested using the LAL method, which indicated that the level of endotoxin units (EU) per microgram (μg) was less than 0.1.

Activity

The Recombinant Human PTN has been proven to be completely biologically active when compared to standard. To test its activity, E16-E18 rat embryonic cortical neurons were used and it was observed that the Recombinant Human PTN was effective in enhancing the neurite outgrowth of these neurons when they were plated on culture plates that had been pre-coated with a solution of 5-10µg/ml of the protein. This test proves that the Recombinant Human PTN is a potent factor in promoting the growth of neurons, which is a vital function for the development and maintenance of the nervous system.

Reconstitution

Prior to opening this vial, we suggest gently centrifuging it to bring the materials to the bottom. To prepare, reconstitute in sterile distilled water or an aqueous buffer that contains 0.1% BSA to reach a concentration of 0.1-1.0 mg/mL. Make sure to divide stock solutions into working aliquots and place them in storage at or below -20°C. When creating further dilutions, use appropriate buffered solutions.

Stability & Storage

To ensure stability, it is recommended to store this lyophilized preparation at a temperature between 2-8 °C. However, for long term storage, it should be kept at -20 °C, preferably in a desiccated state. When reconstituted, the preparation remains stable for up to one week when stored at 2-8 °C. To maximize its stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P21246

Gene IDs

5764