Recombinant Rat SDF-1α/CXCL12α #abs01195

abs01195-2ug

1-2 Weeks

138

abs01195-10ug

1-2 Weeks

344

abs01195-1mg

1-2 Weeks

3891

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

VCLSTKLHII EPVCYQASKC PSGNFAKKLI LDCPVQIKRN TLSRPKCNSI YALARKVVQI SHARELSNDK KPWLKYVRNC.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method confirms that the amount of rRtSDF-1α/CXCL12α is less than 1 EU/μg. To create similar content, the information from the original text needs to be rearranged without changing its meaning.

Activity

The standard and our product exhibit comparable biological activity. Our product's biological activity has been verified through a chemotaxis bioassay on human peripheral blood monocytes, where it was found to be fully active at concentrations ranging between 50-100 ng/ml.

Reconstitution

To ensure proper handling, we suggest briefly centrifuging the vial before opening it to settle the contents at the bottom. Reconstitute the substance in either sterile distilled water or an aqueous buffer with 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/mL. For long-term storage, divide the stock solutions into working aliquots and store them at or below -20 °C. When making further dilutions, utilize appropriate buffered solutions.

Stability & Storage

For optimal storage conditions, it is recommended to keep this lyophilized preparation at -20 °C. However, it can also be stored at 2-8 °C without compromising its stability. To ensure that the preparation remains intact, it is advisable to keep it desiccated. Once reconstituted, it can be stored for up to one week at 2-8 °C. For long-term storage, it is recommended to divide the reconstituted preparation into smaller aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

Q9QZD1

Gene IDs

24772

References

References

1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.

2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.

3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.

4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.

5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.

6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.

7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.