Recombinant Rat SDF-1β/CXCL12β #abs01196

abs01196-2ug

1-2 Weeks

138

abs01196-10ug

1-2 Weeks

344

abs01196-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution was filtered through a 0.2 μm filter and then lyophilized to obtain a highly pure and stable product. The solution was made up of 20 mM PB, pH 7.4, and 150 mM NaCl, ensuring optimal conditions for the product's stability. The resulting lyophilized product is ready for use in a variety of applications.

Properties

aa_sequence

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"Silvery sparks flash across the night sky, casting an ethereal glow on the surrounding forest. A mysterious figure sneaks quietly through the trees, their footsteps barely audible on the soft earth. The crisp cool air sends a shiver down their spine as they make their way towards their destination, a secret location known only to a select few. With each step, their heart beats faster, and their anticipation grows. When they finally arrive at the hidden clearing, they are greeted by a group of others who share their passion for adventure and thrilling experiences."

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The level of rRtSDF-1β/CXCL12β is below 1 EU/μg, which was verified by the LAL method. It is important to note that this information is based on the original text.

Activity

The standard and the sample showed comparable biological activity, with the sample fully maintaining its biological activity. To determine biological activity, a chemotaxis bioassay was conducted using human peripheral blood monocytes. The concentration range of the sample that exhibited biological activity was found to be 100-200 ng/ml.

Reconstitution

Please ensure that this vial is briefly centrifuged before opening in order to settle the contents at the bottom. To create a solution, reconstitute the contents in either sterile distilled water or an aqueous buffer containing 0.1 % BSA, aiming for a concentration of 0.1-1.0 mg/mL. It is recommended to divide stock solutions into smaller working aliquots and store them at or below -20 °C. Any further dilutions should be made using appropriate buffered solutions.

Stability & Storage

To ensure optimal stability, it is recommended to store this lyophilized preparation at -20 °C for long-term storage in a desiccated state. For short-term storage, a temperature range of 2-8 °C is suitable. Once reconstituted, the preparation remains stable for one week when stored at 2-8 °C. To maximize its stability, it is advised to divide the reconstituted preparation into working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

Q9QZD1

Gene IDs

24772

References

References

1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.

2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.

3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.

4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.

5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.

6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.

7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.