Recombinant Mouse LIX/CXCL5 #abs01158

abs01158-5ug

1-2 Weeks

138

abs01158-20ug

1-2 Weeks

344

abs01158-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

QEVNPIKALI LPTVCLTVAC AKNPILINVV EVITVPACQP TIKAKLNVCE QKDAPLCEVK PRVIITKKIQ GKKSAKDLKR AEVASLATRN VPQSSVIAET.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The concentration of rMuLIX/CXCL5, as detected using the LAL method, is found to be lower than 1 EU/μg. Let me provide you with a rephrased version of the given information, ensuring that the content remains consistent with the original text:
The LAL method analysis reveals that the amount of rMuLIX/CXCL5 present is less than 1 EU/μg.

Activity

The standard and the fully biologically active form exhibit comparable properties. The concentration of 10-100 ng/ml is determined through a chemotaxis bioassay, which measures the biological activity using human peripheral blood neutrophils. Let's discuss the content in a different manner without following the method of generating content as done by ChapGPT.

Reconstitution

To ensure the contents are properly settled, it is recommended to briefly centrifuge the vial before opening. Prepare a solution with a concentration of 0.1-1.0 mg/mL by reconstituting in sterile distilled water or an aqueous buffer containing 0.1% BSA. Divide the stock solutions into smaller aliquots for immediate use and store them at a temperature not exceeding -20 °C. Dilute further as necessary using suitable buffered solutions. Please note that the generated content is based on the original text information, but presented in a different manner.

Stability & Storage

For long-term storage, it is recommended to keep this lyophilized preparation at -20 °C, preferably in a desiccated condition. However, it can also remain stable at 2-8 °C. Once reconstituted, it can be stored at 2-8 °C for up to one week. To ensure optimal stability, it is advisable to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P50228

Gene IDs

20311

References

References

1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82.

2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.

3. Wuyts A, Haelens A, Proost P, et al. 1996. J Immunol. 157:1736-43.

4. Wuyts A, D'Haese A, Cremers V, et al. 1999. J Immunol. 163:6155-63.