Recombinant Rat Fractalkine/CX3CL1 #abs01200

abs01200-5ug

1-2 Weeks

138

abs01200-20ug

1-2 Weeks

344

abs01200-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

pH 7.4, the concentrated solution was filtered through a 0.2 μm filter and subsequently lyophilized. The lyophilization process involved removing the solvent from the 2× PBS solution while maintaining the pH at 7.4.

Properties

aa_sequence

ALKMTIMTSK HQLICGTVNP EPVIHNYLQL NTIKHKRCGA QLIETHRRFC DPEVCDQWAK AQHMDHLTAL RNGLTQ. One possible rearrangement of the original text could be: "ALKMTIMTSK HQLICGTVNP EPVIHNYLQL NTIKHKRCGA QLIETHRRFC DPEVCDQWAK AQHMDHLTAL RNGLTQ."

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the rRtFractalkine/CX3CL1 contains less than 1 EU/μg. Can you please create a similar statement using different sentence structure while maintaining the factual information?

Activity

The standard and the tested sample exhibit similar biological activity, with the latter being fully biologically active. The determination of biological activity was based on a chemotaxis bioassay involving human monocytes. It was found that the concentration required to achieve the same activity as the standard was between 5.0-10 ng/ml.

Reconstitution

To ensure optimal results, it is recommended to briefly centrifuge the vial before opening to ensure the contents settle at the bottom. Reconstitute the product in sterile distilled water or an aqueous buffer containing 0.1% BSA, to achieve a concentration of 0.1-1.0 mg/mL. Stock solutions should be divided into working aliquots and stored at -20 °C or below. Dilutions should be made in appropriate buffered solutions.

Stability & Storage

The lyophilized preparation is capable of remaining stable when stored within the temperature range of 2-8 °C. However, for long-term storage, it is recommended to keep it at -20 °C, preferably in a desiccated state. Once the preparation is reconstituted, it can retain stability for up to one week when stored at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller, working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles in order to maintain its quality.

Target

Accession

O55145

Gene IDs

89808

References

References

1. Umehara H, Bloom ET, Okazaki T, et al. 2004. Arterioscler Thromb Vasc Biol. 24:34-40.

2. Umehara H, Tanaka M, Sawaki T, et al. 2006. Mod Rheumatol. 16:124-30.

3. Bazan JF, Bacon KB, Hardiman G, et al. 1997. Nature. 385:640-4.

4. Mizoue LS, Bazan JF, Johnson EC, et al. 1999. Biochemistry. 38:1402-14.