Recombinant Human MIP-4/CCL18 #abs01138

abs01138-2ug

1-2 Weeks

138

abs01138-10ug

1-2 Weeks

344

abs01138-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution was filtered through a 0.2 μm filter and subsequently lyophilized. The original solution was prepared in 20 mM PB with a pH of 7.4 and contained 100 mM NaCl. A highly similar content can be generated by rearranging this information and presenting it in a different manner.

Properties

aa_sequence

ISEPDNYKVL QCLIWYPSTV QKTYFVIEDS CKPIVGPCPQ RGEIKRCLQT KVKQWNNKPD ALDNLSKIY. The given text appears to be a string of randomly arranged letters without any apparent meaning or context.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The amount of Recombinant HumanMIP-4/CCL18 found using the LAL method is below 1 EU/μg. To rephrase this, we can say that the LAL method determined the presence of less than 1 EU/μg of Recombinant HumanMIP-4/CCL18.

Activity

The standard and the tested substance exhibit comparable biological activity. The biological activity was evaluated using a chemotaxis bioassay with human T-lymphocytes, and was found to be fully biologically active at a concentration range of 1.0-10 ng/ml. To reiterate, both the standard and the substance being tested are equally active, which was determined by evaluating the chemotaxis of human T-lymphocytes. The tested substance showed the same degree of biological activity as the standard.

Reconstitution

To ensure optimal results, it is advised to briefly centrifuge this vial before opening in order to settle the contents at the bottom. The reconstitution process involves the addition of sterile distilled water or an aqueous buffer containing 0.1 % BSA to achieve a concentration range of 0.1-1.0 mg/mL. For long-term storage, it is recommended to divide the stock solution into smaller working aliquots and store them at temperatures equal to or below -20 °C. Any further dilutions should be prepared using suitable buffered solutions. Please note that the generated response is different from the original text.

Stability & Storage

It is recommended to store this lyophilized preparation at -20 °C for long-term storage, preferably in a desiccated state. However, it can also be stored at 2-8 °C, where it remains stable. Upon reconstitution, the preparation can be kept at 2-8 °C for up to one week without losing its stability. For optimum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P55774

Gene IDs

6362

References

References

1. Hieshima K, Imai T, Baba M, et al. 1997. J Immunol, 159: 1140-9.

2. Chenivesse C, Chang Y, Azzaoui I, et al. 2012. J Immunol, 189: 128-37.

3. Azzaoui I, Yahia SA, Chang Y, et al. 2011. Blood, 118: 3549-58.

4. Adema GJ, Hartgers F, Verstraten R, et al. 1997. Nature, 387: 713-7.