Membrane Disrupting Agents
Please note that the price provided is purely for reference purposes. For detailed pricing information, we kindly request you to get in touch with our sales representative, Vecent. It is important to contact Vecent for accurate and specific pricing details.
Description
| SKU-Pack Size | Availability | Price |
| abs9111-150ml | In stock | $90.00 |
Please note that the price provided is purely for reference purposes. For detailed pricing information, we kindly request you to get in touch with our sales representative, Vecent. It is important to contact Vecent for accurate and specific pricing details.
Description | |
| Description | Cellular analysis is a critical aspect of scientific research, and broken requests are an essential tool for intracellular cytokine analysis. These requests assist in processing the cell membrane before dyeing, allowing for complete antibody holes and easy detection of intracellular antigens using flow cytometry instrumentation. The cells are treated with a fixing agent and then ruptured for processing, after which an antibody or detection reagent is added and incubated for a specific period. This product is a convenient film breaker that is ready to use. Its optimized formula reduces non-specific staining, improves the signal-to-noise ratio, and does not affect the cells' integrity. |
| Applications | Flow analysis |
| Usage | The first step in the cell testing process involved harvesting the cells. After harvesting, the cells were subjected to centrifugation at 1000rpm for 10 minutes, and the supernatant was then discarded. In the next step, 500ul of fixative, which had been pre-cooled at 4℃, was added to the cells. It was important to incubate the cells at room temperature while avoiding exposure to light for a duration of 10 minutes. Following the incubation period, another round of centrifugation at 1000rpm for 10 minutes was performed. The resulting supernatant was then discarded, and the cells were washed once with PBS. To achieve this, the cells were centrifuged again at 1000rpm for 10 minutes, and the supernatant was discarded. Subsequently, 1.5mL of film breaker was added to the cells. The cells were incubated at room temperature for a period of 10-15 minutes. Once the incubation was complete, centrifugation was carried out at 1000rpm for 5 minutes. The supernatant was discarded, and an appropriate amount of film breaker was added to resuscitate the cells. Finally, after staining the cells with the detection antibody, they were analyzed using flow cytometry. |
Overview | |
| Storage Temp. | Stored at 4℃ for one year |
Properties | |
| Synonym | Permeabilization Wash Buffer |
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