Fixatives

Here is a rearranged version of the provided content with similar information:
To begin the cell testing process, the cells should first be centrifuged at 1000rpm for 10 minutes. Once the centrifugation is complete, carefully remove and discard the supernatant. Next, add 500ul of fixative that has been pre-cooled to 4℃. Allow the cells to incubate at room temperature, making sure to keep them away from light, for a duration of 10 minutes.
After the incubation period, it's time for another round of centrifugation at 1000 RPM for 10 minutes. As before, dispose of the supernatant and proceed to add PBS for a gentle wash. Centrifuge the cells once more at 1000 RPM for 10 minutes to ensure proper washing.
Once the supernatant has been discarded, it's now necessary to add 1.5ml of a film breaker solution. Let the cells incubate at room temperature for a period of 10-15 minutes. After the incubation, conduct another round of centrifugation, this time for 5 minutes at 1000 RPM.
Getting rid of the supernatant once again, it's time to resuscitate the cells by adding an appropriate amount of film breaker. Make sure to carefully determine the required quantity for optimal results.
To complete the process, the cells should be stained with the detection antibody and then analyzed using flow cytometry.

4℃ to avoid light, valid for 1 year;

Fixation Buffer