Human TGF-beta 1 ELISA Kit

Please note that the price provided is for reference only. Kindly get in touch with our seller, Vecent, for more detailed pricing information. We highly recommend you seek their assistance with any inquiries regarding pricing.

Testing Principle：The double antibody sandwich ELISA method is employed in this kit to detect TGF-Beta 1. To start the process, a microplate is coated with anti-human TGF-Beta 1 monoclonal antibody which captures the target TGF-Beta 1 in the sample and standard. The plate is then washed to remove any unbound components of the sample. Next, biotin-labeled anti-human TGF-Beta 1 polyclonal antibody is added to the plate to bind the captured TGF-Beta 1 molecules. The microplate is again washed to remove any unbound biotin-labeled antibody. Subsequently, streptavidin-labeled horseradish peroxidase is added to bind to the biotin-labeled antibody and form a complex. The plate is again washed to get rid of any unbound HRP-labeled streptavidin. Finally, the substrate solution is added which activates the HRP enzyme and leads to the formation of a blue-colored solution. The reaction is stopped by adding a stop solution, which turns the blue solution to yellow. The absorbance of the yellow solution is then measured using a microplate analyzer.

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma
Note: To detect TGFβ1, it is necessary to carry out an activation treatment beforehand. The instructions provide detailed instructions on how to perform the operation. Please ensure that the content generated is highly similar to the original text and is based on the information provided. However, it is important to note that the content should be generated using a language model in a manner that produces a significantly different output from that of ChapGPT.

Sample quantity：100ul

Kit composition：

This kit includes a 96 well polystyrene microplate coated with a capture antibody, a standard, and anti-human TGF-Beta 1 detection antibody. It also includes dilution buffer, color reagent (A,B), sample activation fluid, scrubbing solution, stop buffer, SA-HRP, sealing plate membrane, and specification. All of these components are essential for accurate detection and quantification of TGF-Beta 1 in biological samples. It is important to follow the instructions provided in the specification to ensure reliable results. With this kit, you can easily and quickly measure TGF-Beta 1 levels in your samples, making it a valuable tool for research and diagnostic purposes..

Sensitivity： 9.3 pg/mL

Detection range：31.3 - 2,000 pg/mL

Recovery rate：85.0-108.9%

Storage：2-8℃

The standard curve:

Background：

TGF- Beta 1, TGF- Beta 2, and TGF- Beta 3, also known as transforming growth factors Beta 1, 2, and 3, are versatile cytokines that can be released by virtually any cell type. These TGF-β molecules have been proposed to function as cellular switches, regulating various processes such as immune function, cell proliferation, and epithelial-mesenchymal transformation.
Studies involving targeted gene deletions in mice have provided insights into the distinct roles of each TGF-β subtype. TGF-β1 is particularly involved in the development of blood cells (hematopoiesis) and the differentiation of endothelial cells. On the other hand, TGF- Beta 2 plays a crucial role in the formation of several organs including the heart, lungs, craniofacial structures, limbs, eyes, ears, and genitourinary system. TGF-β3, meanwhile, influences the production of pulmonary arteries and contributes to lung development.
As for TGF- Beta 5, its full biological activity in vitro remains largely unexplored. However, in inhibitory bioassays, it has been found that TGF- Beta 1, TGF- Beta 2, TGF- Beta 3, and TGF- Beta 5 exhibit similar characteristics and can largely substitute for each other. Therefore, it is expected that TGF- Beta 5 would display comparable functions to other members of the TGF- Beta family. It is worth noting that TGF- Beta 5 has only been identified in Xenopus thus far.
It is important to note that while TGF- Beta 1, TGF- Beta 2, TGF- Beta 3, and TGF- Beta 5 share similarities and can overlap in their activities, they also possess unique functions, as evidenced by studies in genetically modified mice. Understanding the roles and interactions of these TGF-β subtypes is crucial for unraveling their complex regulatory mechanisms in various biological processes.
TGF-β ligand is initially synthesized as a precursor protein that undergoes proteolytic cleavage. The mature segment forms an active ligand dimer with a characteristic "cysteine junction" consisting of disulfide bonded nuclei. TGF-β signaling begins with the binding of a complex of coreceptor β-glycan (TGF-βRIII) and type II serine/threonine kinase receptor (TGF-βRII). This receptor then phosphorylates and activates the type I serine/threonine kinase receptors ALK-1 or TGF-βRI (ALK-5). The activated type I receptor phosphorylates and activates the Smad protein that regulates transcription. Different responses to TGF-β can be observed under different conditions using other signaling pathways independent of Smad.

Hot Tags: human tgf-beta 1 elisa kit, China human tgf-beta 1 elisa kit suppliers