Porcine IFN-gamma ELISA Kit

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Cat: abs540001

Size: 96T

Porcine IFN-gamma ELISA Kit

Testing Principle

The enzyme immunoassay technique is utilized in this kit, specifically the quantitative sandwich method. A plate with a high affinity coating of a particular anti-porcine interferon-gamma antibody serves as the substrate. Test samples, standards, and biotinylated detection antibodies are combined to form immune complexes with the IFN-gamma in the samples. The excess material is washed away before horseradish peroxidase-labeled streptavidin is added. Following washing, a chromogenic substrate is added, and the color is allowed to develop in darkness. The reaction is halted by introducing a stop solution and measuring absorbance at 450 nm (with reference correction at 540 nm or 570 nm).

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

The specification includes a 96-well polystyrene microplate that is coated with a capture antibody. It also includes a standard and a detection antibody for use in an ELISA assay. The kit provides a 10×reagent diluent to prepare samples, as well as color reagents A and B for signal detection. Additionally, a 25× wash buffer is included for proper sample washing. To stop the reaction, a stop solution is provided. The kit also comes with ELISA plate sealers to prevent evaporation during incubation.

Sensitivity:  7.8 pg/mL

Detection range:  62.5 - 4,000 pg/mL

Recovery rate: 90-116%

Storage: 2-8 ° C

The standard curve:

Background: IFN-gamma

Interferon Gamma, or type II interferon, is a crucial cytokine that plays a crucial role in modulating the immune system. Originally discovered for its ability to combat viruses, this cytokine has since been found to have far-reaching effects on host defense. Through its antiviral properties, as well as its ability to inhibit cell proliferation and influence immune function, interferon gamma is a vital player in the body's defenses against disease. With its numerous functions, it is clear that interferon gamma is a key component of the immune system that deserves further study and understanding.

The effects of interferon gamma on various cell types are considerable. This cytokine is known to induce the expression of several critical membrane proteins, including MHC-I and MHC-II, Fc receptors, leukocyte adhesion molecules, and B7 family antigens. Additionally, interferon gamma can trigger the activation of macrophages and guide the synthesis, transformation, and secretion of B-cell immunoglobulin. Interferon gamma can also impact T-helper cell subtypes by inhibiting Th2 differentiation and promoting Th1 growth.
One interesting aspect of interferon gamma is its potential involvement in the development of inflammatory diseases like autoimmunity and atherosclerosis. By influencing cytokine production and upregulating the expression of key membrane proteins, interferon gamma may contribute to the pathogenesis of these disorders.
Overall, the effects of interferon gamma are multifaceted and far-reaching. Further research is needed to fully understand its role in health and disease.

Interferon gamma is a dimer with a molecular weight of 20-25 kD, and it is glycosylated to varying degrees. In humans, there is a high amino acid sequence similarity between Interferon gamma and rhesus monkeys, while the similarity with cattle, dogs, horses, cats, and pigs is around 59-64%. Interferon gamma in cotton mice and rats has a low similarity with humans, around 37-43%.

The interferon dimer first binds to the transmembrane receptor IFN-γ RI (α subunit) and then to IFN-γ RII (β subunit) to form a functional complex consisting of two α subunits and two β subunits.IFN-γ RII in the complex receptor can increase ligand affinity and signal transduction efficiency.Although gamma subunits are constitutively expressed in many cell types, the regulatory state of gamma interferon, the receptor expressed by beta subunits, is tightly regulated.

Interferon gamma is secreted by many cells under a range of inflammatory conditions, including dendritic epidermal/γδ T cells, keratinocytes, peripheral bloodγδ T cells, mast cells, neurons, CD8+T cells, macrophages, B cells, neutrophils, natural killer cells, CD4+T cells, and testicular sperm cells.

Technical hints: For research use only, not for in vitro diagnosis.

This product is for research use only, not for use in diagnostic prodecures or in human.

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