Human IL-9 High Sensitivity ELISA Kit

SKU-Pack Size	Availability	Price
abs551806-96T	4 weeks	$580.00

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Testing Principle：

The double antibody sandwich ELISA technique is employed in this kit. The conjugate plate is preloaded with a specific anti-human CXCL13 capture antibody, known for its high affinity. Firstly, standard substance, as well as the sample to be tested and the biotin-labeled detection antibody, are added sequentially into the HRP-plate well. Through thorough mixing and oscillation, the CXCL13 present in the sample undergoes a 2-hour incubation process at room temperature. During this incubation, CXCL13 from the sample binds to both the solid phase antibody and the detection antibody.
In order to remove any unbound or free components, extensive washing is performed. Subsequently, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is introduced. After another round of washing, TMB color substrate is added and allowed to incubate in the dark at room temperature. This step facilitates the development of color, with the intensity of the reaction being directly proportional to the concentration of CXCL13 in the sample.
The reaction is terminated by adding a termination solution, and the absorbance value is measured at a detection wavelength of 450 nm using a microplate analyzer. It is important to note that this measurement is corrected using a wavelength of 570-630 nm.
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Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：The contents of the package include a pre-coated 96-well plate, standards, a single copy of the CXCL13 test antibody, standard diluent, test buffer, TMB color substrate, washing solution, stop solution, SA-HRP, seal plate film, and instructions. These components are carefully arranged to ensure accurate and efficient testing. The pre-coated 96-well plate serves as the testing platform, while the standards provide reference points for analyzing the results. The CXCL13 test antibody is the key element for detecting and quantifying the presence of CXCL13. Diluting the standards and samples with the standard diluent and test buffer helps to ensure accurate measurements. The TMB color substrate produces a color change when the test is positive, allowing for visual detection of the target molecule. The washing solution helps to remove any unwanted substances or residues, ensuring reliable and clear results. The stop solution halts the reaction, providing a stable endpoint for analysis. The SA-HRP is an enzyme conjugate that aids in the measurement process. Finally, the seal plate film provides a secure cover to prevent contamination and maintain the integrity of the testing environment. The included instructions offer guidance on how to perform the test accurately and interpret the results effectively.

Sensitivity：0.16 pg/mL

Detection range：0.63-40 pg/mL

Recovery rate： 98-116%

Storage：2-8℃

The standard curve：

 

Background：

IL-9 is a type of protein that weighs 14 kDa and is made up of glycosylated molecules. The protein is comprised of 10 cysteine residues that are critical for maintaining the structure through disulfide bonding. The protein is categorized as a cytokine that's produced by T cells, specifically CD4+ helper cells. This protein is essential for regulating various hematopoietic cells by inducing cell proliferation and preventing cell apoptosis. When IL-9 binds to its receptor, it activates several signal transduction and activation (STAT) proteins that facilitate a broad range of biological processes.
Research reveals that the IL-9 gene is a likely candidate in the development of asthma. Studies conducted on mice suggest that this cytokine plays a crucial role in the pathogenesis of bronchial hyperresponsiveness. Additionally, the existence of an IL-9-mediated autocrine loop heightens the possibility of malignancies such as Hodgkin's disease. Enzyme-linked Immunosorbent Assays (ELISA) have shown that large aplastic lymphoma cells, Hodgkin's lymphoma cells, and Lee-Scherer cells can express IL-9. However, non-Hodgkin's lymphoma and peripheral T-cell lymphoma cells don't express this cytokine.

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