Trehalase Microplate Assay Kit

TREH, also known as alpha,alpha-trehalase, is an enzyme with the EC number 3.2.1.28. Its main function is to catalyze the hydrolysis of trehalose, a disaccharide consisting of two glucose molecules joined by an alpha, alpha-1,1 glycosidic bond.
Trehalose is a sugar found in many organisms, including bacteria, fungi, plants, and animals. It is used as an energy source, a protective agent against stress, and a signaling molecule. Trehalase is important because it breaks down trehalose into glucose, which can then be used by the cell for energy.
The alpha,alpha-trehalase enzyme is found in various tissues, including the small intestine, liver, pancreas, and kidneys. It has been extensively studied in bacteria and yeast, but its function in higher organisms is still being investigated. Mutations in the TREH gene have been associated with glycogen storage disease type XI, a rare genetic disorder.
In summary, TREH is an enzyme that plays a crucial role in the metabolism of trehalose. It is important for energy production and stress response in many organisms. Its function in higher organisms, including humans, is still not fully understood, but its significance as a genetic marker for certain diseases is evident.

Quantification and Detection of Trehalase Activity is a process that involves measuring and identifying the level of activity of the enzyme trehalase. This enzyme is responsible for the hydrolysis of trehalose into glucose molecules. The detection and quantification of trehalase activity play a crucial role in various scientific and medical studies.
To perform this procedure, several methods can be employed. One common technique is the use of a specific substrate that is acted upon by trehalase and produces a measurable product. By monitoring the formation of this product over a specific time period, the activity of trehalase can be determined.
Another approach is the use of molecular probes or antibodies that specifically bind to trehalase. By attaching a fluorescent or enzymatic marker to these probes, the activity of trehalase can be assessed through the measurement of signal intensity.
Furthermore, some assays utilize genetic techniques to quantify trehalase activity. These methods involve the measurement of trehalase gene expression or the activity of trehalase-related genes in cells or tissues.
The quantification and detection of trehalase activity have wide-ranging applications. In the field of biology, it can aid in understanding the role of trehalase in various physiological processes such as carbohydrate metabolism and stress response. Additionally, it can be used in the diagnosis and monitoring of metabolic disorders related to trehalose metabolism, such as glycogen storage diseases.

0.5 mmol/L - 5 mmol/L

Shipped and store at 4°C for 6 months.