Aspartate Transaminase Microplate Assay Kit

Aspartate transaminase, also known as AST, is an enzyme that plays a crucial role in the metabolism of amino acids. It is also referred to as aspartate aminotransferase, glutamic-aspartic transaminase, glutamic-oxaloacetic transaminase, or Transaminase A. This enzyme catalyzes the transfer of an amino group from aspartate to an alpha-keto acid, producing oxaloacetate and glutamate. It is primarily found in the liver, heart, skeletal muscles, and kidneys, with lower levels present in the brain and other organs. AST is commonly measured in blood tests to assess liver function and diagnose certain medical conditions, such as liver disease or myocardial infarction.

Aspartate transaminase activity can be detected and quantified using various methods. By rearranging the original content and providing information based on the detection and measurement of aspartate transaminase activity, a similar but distinct piece of content can be generated.
The accurate measurement of aspartate transaminase (AST) activity is essential in diagnosing and monitoring various medical conditions. There are several techniques available for detecting and quantifying AST activity in biological samples.
One common method used for detecting AST activity is spectrophotometry. This technique involves measuring the absorption of light at a specific wavelength by a reaction product formed during the enzymatic reaction catalyzed by AST. The higher the enzyme activity, the more intense the color development will be, allowing for quantification.
Another approach to measure AST activity is by using fluorometry. In this method, a fluorogenic substrate is utilized, which upon reacting with AST produces a fluorescent product. The intensity of the fluorescence emitted is directly proportional to the enzyme activity, enabling accurate quantification.
Additionally, enzyme-linked immunosorbent assay (ELISA) can be employed to measure AST activity. This technique involves using specific antibodies that recognize and bind to AST, allowing for the quantification of the enzyme. ELISA provides high sensitivity and specificity in detecting AST activity.
Furthermore, more advanced techniques such as mass spectrometry can also be utilized to measure AST activity. Mass spectrometry allows for the identification and quantification of specific enzymatic products, providing highly accurate results.
It is important to note that these methods may require proper sample preparation and quality control measures to ensure reliable and reproducible results. Utilizing appropriate standards and controls is crucial for accurate quantification of AST activity.
In conclusion, the detection and quantification of aspartate transaminase activity are essential in various medical applications. Spectrophotometry, fluorometry, ELISA, and mass spectrometry are some of the techniques available for measuring AST activity. Proper sample preparation, quality control, and the use of standards are crucial for obtaining reliable and accurate results.

0.625 umol/ml - 20 umol/ml

Shipped and store at 4°C for 6 months.