Human TNF-α High Sensitivity ELISA Kit

SKU-Pack Size	Availability	Price
abs551818-96T	In stock	$580.00

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Testing Principle：

The double antibody sandwich ELISA technique is employed in this kit. The conjugate plate is preloaded with a specific anti-human CXCL13 Capture Antibody, which exhibits high affinity. To begin the assay, the standard substance, the sample being tested, and the biotin-labeled detection antibody are added sequentially into the HRP-plate well. The mixture is thoroughly oscillated and mixed, allowing for a 2-hour incubation period at room temperature. During this incubation step, the CXCL13 in the sample combines with the solid phase antibody and the detection antibody. After incubation, the plate is thoroughly washed to eliminate any unbound or free components. Next, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added to the plate. Following another round of washing, a TMB color substrate is introduced and allowed to incubate in the dark at room temperature for color development. The depth of the color reaction is directly correlated to the concentration of CXCL13 present in the sample. To terminate the reaction, a termination solution is added, and the absorbance value is measured at a detection wavelength of 450 nm using a microplate analyzer. It is important to note that during measurement, a correction wavelength of 570-630 nm is utilized. By rearranging the provided information, the following content is generated while ensuring its proximity to the original text.

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：Included in this kit is a pre-coated 96-well plate along with standards, a single copy of the CXCL13 test antibody, standard diluent, test buffer, TMB color substrate, washing solution, stop solution, SA-HRP, seal plate film, and instructions. The content has been arranged to provide all of the necessary materials for conducting accurate and reliable testing. With this kit, you will have everything needed to carry out your testing procedures efficiently and with confidence. The instructions provided will guide you through the process step-by-step, ensuring that you produce reliable results every time.

Sensitivity：0.16 pg/mL

Detection range：1.56-100 pg/mL

Recovery rate：100-108%

Storage：2-8℃

The standard curve：

 

Background：

Tumor necrosis factor α (TNF-α), also referred to as cachexin and TNFSF1A, is an adipokine that participates in systemic inflammation and acts as a stimulator of acute phase responses, alongside other cytokines. Its primary source of production is activated macrophages, although it can also be secreted by various cell types including CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNF-α possesses diverse regulatory functions in immune responses but can also serve as a potential pyrogen.
Upon encountering stimuli such as infectious agents or tissue damage, TNF-α is released into circulation. Its actions include activating neutrophils, modifying the characteristics of vascular endothelial cells, regulating metabolic activity in different tissues, and even demonstrating tumor-killing properties by inducing localized coagulation. Of particular significance, TNF-α plays a crucial role in the development of inflammation in joint tissues and other bodily structures. Furthermore, emerging evidence suggests its involvement in the pathogenesis of AIDS, broadening its relevance beyond the realm of inflammation.
The measurement of TNF-α levels also proves valuable in transplantation studies, providing insights into the underlying processes. Overall, TNF-α serves as a multifaceted molecule, central to immune responses, inflammation, and potentially contributing to various disease pathways.

 

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