PI Cell Cycle And Apoptosis Kit

PI Cell Cycle and Apoptosis Kit

This particular product is designed for the analysis of cell cycle and apoptosis using Propidium iodide (PI) staining. Propidium iodide is a type of dye that binds to double-stranded DNA. Once incorporated into the DNA, it emits fluorescence, and the intensity of the fluorescence is directly proportional to the amount of double-stranded DNA present. In a normal cell cycle, the different phases have distinct DNA content: G0 and G1 phases have one set of chromosomes, G2 and M phases have two sets of chromosomes, and the S phase is the phase where DNA replication occurs. By staining cells with PI, the fluorescence intensity can vary, allowing differentiation between cells in different phases of the cell cycle. For instance, cells in G0 and G1 phases exhibit a fluorescence intensity of 1, while cells in G2 and M phases have a fluorescence intensity of 2. Cells in the S phase will display a fluorescence intensity somewhere between 1 and 2.
Moreover, PI staining can also be utilized to detect apoptosis, a process characterized by cell death. During apoptosis, the nucleus shrinks and DNA fragments, resulting in the loss of DNA from the permeable cell membrane during staining. Consequently, when analyzed by flow cytometry, the fluorescence intensity of apoptotic cells will be less than 1, forming a distinct sub-G1 peak. Another way to measure apoptosis is by examining changes in light scattering properties of cells through flow cytometry. In the early stages of apoptosis, chromatin condenses, leading to an increase in cell density and a noticeable decrease in forward angular light scattering. Later in the apoptotic process, the formation of apoptotic bodies occurs, bringing about a significant reduction in both forward and lateral angular light scattering. The components included in this product are Propidium Iodide (500 μl), BRNase A (500 μl), CStaining Buffer (25ml), in a total volume of 50TA. By using this kit, researchers can accurately analyze cell cycle progression and apoptosis based on the fluorescence and scattering properties of stained cells.

Store at -20° C away from light for 12 months.

During operation, it is crucial to prioritize protection against the toxicity of PI. Take necessary precautions such as wearing protective eyewear, avoiding inhalation, and using disposable gloves.
When storing and using PI, it is important to be cautious and prevent exposure to light as it can lead to a phenomenon called quenching. Take utmost care to avoid any illumination during storage and usage.