Na+/K+ ATPase Microplate Assay Kit

EC 3.6.3.9 is a prominent enzyme known as Sodium/potassium-exchanging ATPase. This enzyme, also called Na(+)/K(+)-ATPase or Na(+)/K(+)-exchanging ATPase, plays a crucial role in cellular processes. It is commonly referred to as the sodium pump or Sodium/potassium-transporting ATPase due to its ability to transport sodium and potassium ions across cell membranes.
The function of this ATPase is to maintain the concentration gradients of sodium and potassium ions across the membrane. It accomplishes this by hydrolyzing ATP to provide the necessary energy for the active transport of three sodium ions out of the cell and two potassium ions into the cell. This transport is essential for various physiological processes, such as nerve impulse transmission, muscle contraction, and osmoregulation.
The sodium/potassium-exchanging ATPase is a transmembrane protein composed of multiple subunits. It consists of a catalytic alpha subunit, which possesses ATP-binding and phosphorylation sites, and a regulatory beta subunit. The alpha subunit is responsible for ion translocation, while the beta subunit modulates the enzyme's activity and localization.
Mutations or dysregulation of the sodium/potassium-exchanging ATPase can lead to various diseases and disorders. For example, disruptions in its function have been implicated in neurological disorders like Alzheimer's disease, epilepsy, and bipolar disorder. Additionally, the sodium pump has been targeted by certain drugs that regulate blood pressure and cardiac function.
In summary, the sodium/potassium-exchanging ATPase, also known as Na(+)/K(+)-ATPase, is a vital enzyme involved in the active transport of sodium and potassium ions across cell membranes. Its role in maintaining ion gradients is crucial for numerous physiological processes, and dysregulation of this enzyme can contribute to various diseases and disorders.

Measuring the activity of Na+/K+ ATPase is essential for studying cellular signaling and metabolism. It is important to accurately detect and quantify the levels of this enzyme in order to understand its role in maintaining homeostasis in cells. One common method used for this purpose involves spectrophotometric assays, which measure the amount of phosphate released by ATP hydrolysis. These assays are highly sensitive and reliable, providing a quantitative measure of Na+/K+ ATPase activity. Other methods, such as radiometric assays and immunological techniques, can also be used to detect and quantify Na+/K+ ATPase activity, but they may have limitations in terms of accuracy or accessibility. Overall, determining Na+/K+ ATPase activity is crucial for understanding the physiological processes that are dependent on this enzyme and for developing therapeutic interventions to treat related disorders.

0.01 umol/ml - 5 umol/ml

Shipped and store at 4°C for 6 months.