Donkey Anti-Goat IgG-Cy3 #abs20028

abs20028-100ul

In Stock

148

abs20028-500ul

In Stock

586

Description

Immunoaffinity chromatography is used to separate intact whole IgG antibodies from antisera, retaining their Fc and Fab portions linked by disulfide bonds. This results in a divalent form with an average molecular weight of approximately 160 kDa. The whole IgG variant is widely applicable in immunodetection procedures and offers a cost-effective solution. It provides the necessary binding capability for various assays while maintaining its structural integrity.

According to immunoelectrophoresis and/or ELISA, the antibody displays reactivity towards the intact molecular structure of goat IgG. It also exhibits reactivity towards the light chains of other goat immunoglobulins, while no reactivity is observed towards non-immunoglobulin serum proteins. The antibody was subjected to ELISA and/or solid-phase adsorption procedures to minimize potential cross-reactivity with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit and rat serum proteins, although it may exhibit some cross-reactivity with immunoglobulins from other species.

Source

Donkey

Specificity

IgG (H+L)

Species Reactivity

Goat

Application

Flow Cytometry, Histo-/Cyto-Chemistry, IF 1:100 - 1:800 are advanced techniques used in the field of molecular biology and cellular analysis. These techniques allow scientists to study the distribution and localization of biomolecules within cells and tissues.
Histo-/Cyto-Chemistry is a branch of biochemistry that involves studying the chemical composition of cells and tissues. It involves the use of specific stains and dyes to visualize specific molecules or cellular structures. This technique provides valuable information about the localization and abundance of molecules within a cell or tissue sample.
Flow Cytometry, on the other hand, is a powerful technique used to analyze and quantify the characteristics of individual cells in a heterogeneous population. It involves staining cells with fluorescent dyes and passing them through a flow cytometer, which measures the fluorescence emitted by each cell. This technique provides information about the size, granularity, and protein expression of individual cells within a population.
In Immunofluorescence (IF) assays, specific antibodies are used to label target proteins within cells or tissues. The antibodies are labeled with fluorescent dyes, which emit light upon binding to their target proteins. IF assays can be used to visualize the localization and distribution of specific proteins within cells or tissues. The dilution factor for IF assays typically ranges from 1:100 to 1:800, depending on the specific experimental requirements.
Overall, these techniques are essential tools in molecular biology and cellular research, allowing scientists to analyze and understand complex biological processes at the cellular and molecular level.

Properties

Concentration

1mg/ml

Clonality

Polyclonal Antibody

Isotype

Whole IgG

Stability & Storage

Store at -20 °C for one year.

Storage buffer

0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
A solution containing 15 mg/ml of Bovine Serum Albumin, which is free from both IgG and protease, is available.
0.05% Sodium Azide, 50% glycerol.

Conjugate

Cy3