Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-ZP2 Polyclonal Antibody#abs139542

Please note that the provided price is only for your reference. For accurate pricing details, please reach out to our seller, Vecent. We suggest contacting Vecent for further information rather than relying solely on the above content. Western blot analysis ZP2 using COLO205 whole cell lysates...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs139542-100ug	1-2 Weeks	301.0
abs139542-50ug	1-2 Weeks	201.0

Overview
catalog	abs139542
Other names	This is information about the processed zona pellucida sperm-binding protein 2, also known as the zona pellucida glycoprotein 2 or ZP2. This protein is a component of the zona pellucida, an extracellular matrix that surrounds mammalian oocytes and plays a key role in fertilization. ZP2 is necessary for sperm binding to the zona pellucida, which is essential for successful fertilization. Other names for this protein include zona pellucida protein A and Zp-2, and it is also known by the gene name ZP2_HUMAN or ZPA.
Source	Rabbit
Specificity	The ZP2 Antibody is capable of identifying total ZP2 levels that occur naturally within the body. By rearranging the content of the original text, we can create highly similar but unique statements that also convey the same information.
Species Reactivity	Human;Monkey
Antigen	ZP2
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human ZP2.
MW	82 kDa
Properties
Concentration	1mg/ml
purification	Utilizing SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. The highly effective technique allowed for the purification process to be carried out efficiently, resulting in a high-quality final product.
Clonality	Polyclonal Antibody
Stability & Storage	One year of storage is recommended at -20 °C to maintain product quality. It is important to avoid subjecting the product to repeated freeze/thaw cycles.
Storage buffer	The rabbit IgG is stored in a solution consisting of phosphate buffered saline with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. The stability of the product is guaranteed for a period of 12 months from the date of receipt.
Target
Background	The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
Posttranslational modification	Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.Proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL exocytosed from cortical granules after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy. Additional proteolytically cleavage of the N-terminal peptide of 30 kDa occurs in one-cell and two-cell embryos.N-glycosylated.O-glycosylated; contains sulfate-substituted glycans.
Celluar localization	Endoplasmic reticulum;Endosome;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;
UniPort	Q05996