Rabbit Anti-HNF4alpha /gamma Polyclonal Antibody #abs130404

abs130404-50ug

1-2 Weeks

201

abs130404-100ug

1-2 Weeks

301

Description

HNF4 alpha is a nuclear transcription factor that functions as a homodimer to bind DNA. Its role encompasses the control of various genes, including hepatocyte nuclear factor 1 alpha, which plays a fundamental role in regulating the expression of hepatic genes like apolipoprotein CIII and alpha 1-antitrypsin, as well as the transthyretin gene. Moreover, it might play a vital part in the development of the liver, kidney, and intestine. Individuals with mutations in HNF4 alpha gene have been linked to monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I.

Other names

Hepatic nuclear factor 4 alpha, also known as HNF-4 alpha or HNF4A, is a transcription factor that plays a crucial role in regulating gene expression in the liver. It is part of the nuclear receptor subfamily 2 group A and is involved in various biological processes related to hepatocyte function and metabolism.
HNF-4 alpha is a key regulator of hepatic gene expression, controlling the transcription of numerous genes involved in glucose metabolism, lipid metabolism, and drug metabolism. It functions by binding to specific DNA sequences in the promoter region of target genes and activating their transcription.
Mutations in the HNF4A gene have been associated with maturity-onset diabetes of the young type 1 (MODY1), a form of diabetes characterized by early-onset and impaired glucose regulation. These mutations disrupt the function of HNF-4 alpha, leading to abnormalities in insulin secretion and glucose metabolism.
HNF-4 alpha is also expressed in other tissues, including the pancreas, intestine, kidney, and ovary, where it plays important roles in regulating gene expression and organ development.
In addition to HNF-4 alpha, there is another isoform called HNF-4 gamma, encoded by the HNF4G gene. HNF-4 gamma shares high structural similarity with HNF-4 alpha and has similar functions in regulating gene expression, particularly in the pancreas and intestine.
Overall, the hepatic nuclear factor 4 alpha and gamma are critical transcription factors that play essential roles in maintaining liver function, glucose homeostasis, and overall metabolic balance in the body.

Source

Rabbit

Specificity

The detection of endogenous levels of HNF4alpha/gamma is possible using the HNF4alpha/gamma Antibody.

Species Reactivity

Human;Mouse;Rat

Application

WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Immunogen

A novel peptide, obtained from human HNF4alpha/gamma, has been synthesized. The synthesized peptide is designed to closely resemble the original sequence, and it is based on the information derived from human HNF4alpha/gamma. Let's discuss this peptide in a different way, diverging from the typical content generation approach employed by ChapGPT.

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the quality and stability of the product, it is advised to keep it stored at a temperature of -20 °C for a duration of one year. In order to avoid any potential damage caused by repeated freezing and thawing, it is recommended to prevent this process from occurring.

Storage buffer

The rabbit IgG is formulated in a phosphate buffered saline solution with a pH of 7.4 and contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store the product at -20 °C for optimal stability, and it has been proven to be stable for up to 12 months from the date of receipt.

Target

Background

A crucial transcription factor that controls gene expression by binding to specific DNA regions is involved in the transcription of various genes including alpha 1-antitrypsin, apolipoprotein CIII, transthyretin, and HNF1-alpha. Its role in the development of the liver, kidney, and intestine is believed to be indispensable. By modulating gene expression, this transcription factor plays a vital role in regulating the cellular processes underlying these organs' development and function.

Posttranslational modification

Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.Acetylation at Lys-458 lowers transcriptional activation by about two-fold.

Celluar localization

Nucleus;

UniPort

P41235/Q14541