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Rabbit Anti-UHRF1 Polyclonal Antibody#abs136449

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Description

Catalog-specification	Delivery time	USD price
abs136449-100ug	1-2 Weeks	301.0
abs136449-50ug	1-2 Weeks	201.0

Overview
catalog	abs136449
Description	UHRF1, also known as ICBP90, NP95, and RNF106, is an E3 ubiquitin-protein ligase candidate. It is believed to play a role in regulating gene expression through methylation. UHRF1 interacts with the inverted 5'-CCAAT-3' box 2 sequence in the promoter region of the TOP2A gene, which leads to the activation of TOP2A expression. This protein is crucial for the transition from the G1 phase to the S phase of the cell cycle and may also be involved in DNA repair and maintaining chromosomal stability. Interestingly, UHRF1's ability to suppress the expression of its direct target genes depends on the binding of its PHD domain to unmodified H3R2 residue on histone proteins. By recognizing this specific histone modification, UHRF1 exerts control over gene regulation mechanisms.
Other names	The E3 ubiquitin-protein ligase UHRF1, also known as hNP95, is a nuclear zinc finger protein that plays a critical role in various cellular processes. It has been identified by different names such as ICBP90, RNF106, TDRD22, and FLJ21925. This protein is involved in the regulation of DNA methylation and histone modifications, which are crucial for the maintenance of genome stability and gene expression. It recognizes the hemimethylated CpG sites and recruits DNMT1, a DNA methyltransferase, for maintenance methylation during DNA replication. Furthermore, UHRF1 is essential for proper cell cycle progression and DNA damage response. It has been implicated in the development of cancer and other human diseases, highlighting its significance as a potential therapeutic target. Overall, UHRF1 is a multifaceted protein with diverse functions that are still being explored. Its potential to serve as a diagnostic marker and therapeutic target warrants further investigation.
Source	Rabbit
Specificity	The endogenous levels of total UHRF1 can be effectively detected using the UHRF1 Antibody. Please note that the content generated below is based on the original text information, but rearranged in a highly similar manner. To detect the total UHRF1 levels present within an organism, the UHRF1 Antibody is utilized. It is capable of identifying the endogenous levels of UHRF1 effectively.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Chicken;Pig;Dog;Sheep;Bovine;
Antigen	UHRF1
Application	The suggested dilutions for using this antibody in various applications are as follows: 1:500-1:2000 for Western blotting (WB), 1:50-1:200 for immunohistochemistry (IHC), 1:20-1:50 for immunofluorescence (IF), 1:20-1:50 for immunoprecipitation (IP), 1:20-1:50 for chromatin immunoprecipitation (ChIP), and 1:20000-1:40000 for ELISA with peptide. These dilutions have been optimized for best results, but optimal dilutions may vary depending on the specific experimental conditions and sample types. It is important to perform proper controls and validate results before interpreting the data.
Immunogen	A synthesized peptide derived from human UHRF1.
MW	91kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.
Tissue specificity	Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
Posttranslational modification	Phosphorylation at Ser-298 of the linker region decreases the binding to H3K9me3. Phosphorylation at Ser-639 by CDK1 during M phase impairs interaction with USP7, preventing deubiquitination and leading to degradation by the proteasome.Ubiquitinated; which leads to proteasomal degradation. Autoubiquitinated; interaction with USP7 leads to deubiquitination and prevents degradation. Ubiquitination and degradation takes place during M phase, when phosphorylation at Ser-639 prevents intereaction with USP7 and subsequent deubiquitination. Polyubiquitination may be stimulated by DNA damage.
Celluar localization	Nucleus;
UniPort	Q96T88