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Rabbit Anti-UBA52 Polyclonal Antibody#abs137498

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Description

Catalog-specification	Delivery time	USD price
abs137498-100ug	1-2 Weeks	301.0
abs137498-50ug	1-2 Weeks	201.0

Overview
catalog	abs137498
Other names	CEP52, also known as 60S ribosomal protein L40, HUBCEP52, or RPL40, is an important protein involved in ribosomal function. It is encoded by the MGC126879 and MGC57125 genes. CEP52 is also known as UBA 52, as it contains a fusion of ubiquitin and a 52-amino acid residue ribosomal protein. Its alternative names include Ubiquitin 60S ribosomal protein L40, Ubiquitin A 52 residue ribosomal protein fusion product 1, and Ubiquitin carboxyl extension protein 52. This protein plays a crucial role in protein synthesis and is essential for the proper functioning of the ribosome.
Source	Rabbit
Specificity	The total UBA52 levels in cells can be detected using the UBA52 Antibody which recognizes the endogenous expression of UBA52. Let's rearrange the above content to generate a highly similar statement: "The endogenous levels of total UBA52 can be detected using the UBA52 Antibody, ensuring accurate detection of UBA52 expression in cells."
Species Reactivity	Human;Mouse;Monkey
Predictive reaction species	Xenopus;Zebrafish;Chicken;Pig;Dog;Horse;Bovine;Sheep;
Antigen	UBA52
Application	： ELISA(peptide) 1:20000-1:40000, WB 1:1000-3000, IF/ICC 1:100-1:500. 。ELISA(peptide)1:20000-1:40000，WB1:1000-3000，IF/ICC1:100-1:500。。
Immunogen	A synthesized peptide derived from human UBA52.
MW	15 kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography was employed to purify the antiserum using SulfoLink™ Coupling Resin. The resin has proven to be an effective means of coupling peptides to support matrices for the purification of target proteins. The resulting purified antiserum can be used for a range of applications including immunodetection and immunoaffinity purification. This method offers a highly specific and efficient purification approach that minimizes sample loss and maximizes yield.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure preservation, the recommended storage condition is -20 °C for a duration of one year. It is important to prevent repetitive freeze/thaw cycles.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, and also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and is stable for a period of 12 months from the date of receipt. To maintain its effectiveness and shelf life, make sure to store it properly. This product is ideal for use in a variety of scientific and research applications, and is guaranteed to provide reliable and consistent results. Order now and experience the benefits of this high-quality product for yourself!
Target
Background	Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Posttranslational modification	Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291). Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30 (PubMed:25527291).
Celluar localization	Cytosol;Endoplasmic reticulum;Endosome;Extracellular region or secreted;Lysosome;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	P62987