Rabbit Anti-Collagen V Alpha1 Polyclonal Antibody#abs140630

Rabbit Anti-Collagen V Alpha1 Polyclonal Antibody#abs140630

Please note that the price mentioned above is provided for your reference only. For the detailed pricing information, kindly get in touch with our sales representative, Vecent. Western blot analysis of extracts from HeLa cells, using Collagen V α1 antibody. This product is for research use only,...

Description

Catalog-specificationDelivery timeUSD price

abs140630-100ug

1-2 Weeks

301.0

abs140630-50ug

1-2 Weeks

201.0

Please note that the price mentioned above is provided for your reference only. For the detailed pricing information, kindly get in touch with our sales representative, Vecent.


Overview

catalog

abs140630
Other namesThe protein known as alpha 1 type V collagen is coded by the COL5A1 gene. It is also referred to as the collagen alpha-1(V) chain or simply as Collagen V. The characteristics and functions of this collagen type make it highly important in maintaining the structural integrity of tissues and organs throughout the body. Its structure and functions can be studied and analyzed in order to gain a better understanding of the mechanisms that underlie various physiological processes.
SourceRabbit
SpecificityThe Collagen V α1 Antibody is capable of identifying the total levels of endogenous Collagen V α1. This indicates that the antibody can detect the presence of Collagen V α1 in the samples under examination.
Species ReactivityHuman
Predictive reaction speciesPig;Sheep;Dog;Horse;Bovine
AntigenCollagen V alpha1
ApplicationThe recommended dilution for WB is between 1:500 to 1:1000, while for IHC it's between 1:50 to 1:200. When it comes to IF/ICC, a dilution of 1:100 to 1:500 is recommended. For ELISA using peptide, a dilution of 1:20000 to 1:40000 is suggested. These dilution ranges are recommended for optimal results and may need to be adjusted for specific experiments. It's important to follow the manufacturer's instructions for the specific antibody being used.
ImmunogenA synthesized peptide.
MW200 KD
Properties

Concentration

1mg/ml

purificationUsing SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. A highly similar content can be generated by rearranging the wording to maintain the same information and meaning while using different phrasing and sentence structure.
ClonalityPolyclonal Antibody
Stability & StorageThe recommended storage condition is -20 °C for a duration of one year. It is important to avoid subjecting the stored material to multiple freeze/thaw cycles.
Storage bufferThe storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that it is stable for a period of 12 months starting from the date of receipt.

Target

Background

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.

Posttranslational modificationProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.Sulfated on 40% of tyrosines.
Celluar localizationEndoplasmic reticulum;Extracellular region or secreted;
UniPortP20908


Western blot analysis of extracts from HeLa cells, using Collagen V α1 antibody.


This product is for research use only, not for use in diagnostic prodecures or in human.


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