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Rabbit Anti-CIDEA Polyclonal Antibody#abs138573

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Description

Catalog-specification	Delivery time	USD price
abs138573-100ug	1-2 Weeks	301.0
abs138573-50ug	1-2 Weeks	201.0

Overview
catalog	abs138573
Other names	CIDE-A, also known as Cell Death Activator CIDE A or Cell Death Inducing DFFA Like Effector A, is a protein that plays a crucial role in cell death processes. It belongs to the family of cell death inducing DNA fragmentation factor, alpha subunit like effectors. CIDE-A, or CIDEA, as it is commonly abbreviated, is involved in regulating apoptosis, a form of programmed cell death. The function of CIDE-A is to initiate and promote the fragmentation of DNA in cells undergoing apoptosis. It acts as a crucial mediator of cell death, providing the necessary signals for the activation of enzymes responsible for DNA fragmentation. CIDE-A can also interact with other proteins involved in apoptosis regulation, further contributing to the progression of programmed cell death. Interestingly, recent studies have linked CIDE-A to metabolic regulation. It has been suggested that CIDE-A may play a role in the regulation of energy balance and lipid metabolism. Research has shown that CIDE-A expression is increased in conditions of obesity and that it may contribute to adipose tissue expansion. Additionally, CIDE-A has been implicated in the modulation of glucose metabolism. The precise mechanisms by which CIDE-A regulates both apoptosis and metabolism remain the subject of ongoing research. However, it is clear that this multifunctional protein plays vital roles in cellular processes related to cell death and metabolic homeostasis. Further understanding of CIDE-A's functions may provide valuable insights into the development of therapeutic approaches for various diseases, including cancer and metabolic disorders.
Source	Rabbit
Specificity	The CIDEA Antibody has the ability to recognize and bind to the total endogenous CIDEA present in the cell or tissue sample. By detecting and quantifying this protein, researchers can gain insight into its role and function in various biological processes. The detection of CIDEA with this antibody is considered highly specific and reliable, making it a valuable tool in scientific studies involving this particular protein.
Species Reactivity	Human
Predictive reaction species	Rabbit;Sheep;Dog;Horse;Bovine
Antigen	CIDEA
Application	The dilution recommendations for the western blot (WB) assay range from 1:1000 to 1:3000, indicating the optimal dilution range for reliable results. On the other hand, the recommended dilution for the immunohistochemistry (IHC) assay is 1:200, ensuring proper staining of the target protein in tissue sections. For the ELISA assay using a peptide as the antigen, it is recommended to perform dilutions ranging from 1:20000 to 1:40000, allowing for accurate detection and quantification of the peptide concentration in the sample. It is essential to follow these dilution guidelines to obtain consistent and reproducible results in these different experimental techniques.
Immunogen	A synthesized peptide derived from human CIDEA.
MW	25 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for the peptide affinity chromatography process to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Make sure to store it at -20 °C for a duration of one year. It is important to avoid subjecting it to multiple freeze/thaw cycles.
Storage buffer	This solution contains Rabbit IgG in a mixture of phosphate buffered saline with a pH of 7.4 and 150mM of NaCl. It also contains 0.02% sodium azide and 50% glycerol for preservation. For optimal storage, it is recommended to keep this solution at a temperature of -20°C. It is also advised to use this product within 12 months following the date of receipt in order to ensure its stability.
Target
Background	Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter (By similarity). Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear.
Tissue specificity	Expressed in omental and subcutaneous adipose tissue (at protein level).
Celluar localization	Cytosol;Mitochondrion;Nucleus;
UniPort	O60543