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Rabbit Anti-TRF1 Polyclonal Antibody#abs140571

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Description

Catalog-specification	Delivery time	USD price
abs140571-100ug	1-2 Weeks	301.0
abs140571-50ug	1-2 Weeks	201.0

Overview
catalog	abs140571
Other names	Possible highly similar content: hTRF1 is also known as Telomeric repeat-binding factor 1 or Telomeric repeat binding protein 1. It plays a crucial role in maintaining the stability of telomeres, which are the protective caps at the ends of chromosomes that shorten with each cell division. Shortened telomeres have been linked to aging, cancer, and other diseases. hTRF1 interacts with a protein called NIMA (Never in Mitosis A)-related kinase 2 (NEK2), also known as NIMA-interacting protein 2 (NIP2) or PIN2. NEK2 is involved in regulating cell division and has been implicated in cancer. The interaction between hTRF1 and NEK2 is thought to help ensure proper mitotic segregation of chromosomes. hTRF1 belongs to the family of telomeric repeat binding factors (TRFs), which bind to the TTAGGG repeats of telomeres. There are two main isoforms of hTRF1, TRF1α and TRF1β, which have different lengths and activities. hTRF1α is the more abundant and active isoform, while hTRF1β may play a more specialized role in cell cycle regulation. hTRF1 has also been shown to interact with other proteins involved in DNA repair and replication, such as ATM, ATR, and DNA polymerase α/primase. These interactions suggest that hTRF1 may have a broader role in DNA metabolism than just telomere protection. Mutations or dysregulation of hTRF1 have been linked to various diseases, including cancer, premature aging syndromes, and bone marrow failure. Therefore, understanding the molecular mechanisms of hTRF1 function and regulation may have important implications for human health.
Source	Rabbit
Specificity	The TRF1 antibody is capable of detecting total TRF1 protein levels that are present naturally in the body. To create similar content, the original text has been reorganized to convey the same information.
Species Reactivity	Human
Antigen	TRF1
Application	For Western blotting, the recommended dilution is 1:1000. As for immunofluorescence or immunocytochemistry, a dilution of 1:100-1:500 is suggested. For ELISA using peptides, the appropriate dilution range is 1:20000-1:40000. It is important to follow these dilution guidelines for optimal results.
Immunogen	A synthesized peptide derived from human TRF1.
MW	50kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum. Please generate a content with high similarity by rearranging the provided information, ensuring it is based on the original text.
Clonality	Polyclonal Antibody
Stability & Storage	To keep your product in optimal condition, it is recommended that you store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this could compromise its quality. Keep in mind that proper storage is crucial for preserving the integrity and effectiveness of your product.
Storage buffer	You can store Rabbit IgG in a solution containing phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. In order to maintain its effectiveness, it's important to keep it at -20°C where it's stable for up to 12 months from the date of receipt.
Target
Background	Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Tissue specificity	Highly expressed and ubiquitous. Isoform Pin2 predominates.
Posttranslational modification	Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Celluar localization	Cytoskeleton;Nucleus;
UniPort	P54274