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Rabbit Anti-TLR2 Polyclonal Antibody#abs136522

Please note that the price provided is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis of extracts of THP-1cell line, using TLR2 antibody. This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs136522-100ug	1-2 Weeks	301.0
abs136522-50ug	1-2 Weeks	201.0

Please note that the price provided is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs136522
Description	TLRs, members of the Toll-like receptor family, are crucial for innate immune responses and are named after the Toll receptor found in Drosophila. These receptors play a significant role in recognizing conserved motifs in pathogens, activating the NF-κB pathway, and regulating immune and inflammatory genes. The TIR domain, a conserved region of approximately 200 amino acids, is shared by both TLRs and members of the IL-1 receptor family. Upon activation, TLRs form associations with various cytoplasmic adaptor proteins that possess TIR domains, such as MyD88, MAL/TIRAP, TRIF, and TRAM. This interaction leads to the recruitment and activation of IRAK1 and IRAK4, which, in turn, form a complex with TRAF6, activating TAK1 and IKK. Consequently, IKK initiates the degradation of IκB, releasing NF-κB from its sequestration within the cytoplasm, and facilitating its activation.
Other names	TLR2, also known as CD282 antigen or TIL4, is a key protein involved in the immune response. It is a member of the Toll-like receptor family and plays a vital role in recognizing and responding to foreign pathogens. The Toll-like receptor 2 precursor, or TLR2_HUMAN, is the initial form of TLR2 before it undergoes proteolytic cleavage to become the mature Toll-like receptor 2. This cleavage process is essential for TLR2 to be functional. Toll/interleukin 1 receptor-like protein 4, or Toll/interleukin 1 receptor-like protein 4, is another name for TLR2. It is called so because of its structural similarity to Toll and interleukin 1 receptors. Overall, Toll-like receptor 2 is a crucial component of the innate immune system. Its activation triggers a signaling cascade that leads to the production of pro-inflammatory cytokines and the initiation of an immune response against invading pathogens.
Source	Rabbit
Specificity	The endogenous levels of total TLR2 can be detected using the TLR2 Antibody. This antibody specifically recognizes and binds to the total TLR2 protein present in the sample, allowing for its detection.
Species Reactivity	Human;Mouse;Rat
Antigen	TLR2
Application	The dilution ratios for WB, IHC, and ELISA (peptide) are as follows: WB - 1:500 to 1:2000, IHC - 1:50 to 1:200, and ELISA (peptide) - 1:20000 to 1:40000. These ratios indicate the recommended dilution factors for each assay. It is important to dilute the samples appropriately in order to achieve optimal results in these experiments. By following these recommended dilution ranges, researchers can ensure accurate and reliable detection of their target of interest.
Immunogen	A synthesized peptide derived from human TLR2.
MW	90kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:21078852, PubMed:17889651). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins (PubMed:10426995, PubMed:10426996). Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6 (PubMed:11441107). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4 (PubMed:16622205). MAPK activation in response to bacterial peptidoglycan also occurs via this receptor (PubMed:16622205). Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36); the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression (PubMed:15809303). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse) (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (PubMed:16880211). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (By similarity).
Tissue specificity	Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
Posttranslational modification	Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
Celluar localization	Golgi apparatus;Plasma Membrane;
UniPort	O60603