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Rabbit Anti-ARF4 Polyclonal Antibody#abs140824

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Description

Catalog-specification	Delivery time	USD price
abs140824-100ug	1-2 Weeks	301.0
abs140824-50ug	1-2 Weeks	201.0

Overview
catalog	abs140824
Other names	ARF2, also known as ADP ribosylation factor 2, and ARF4, also known as ADP ribosylation factor 4, are two important proteins involved in cellular processes. ARF2 and ARF4 play crucial roles in intracellular vesicular trafficking and the regulation of protein trafficking pathways. ARF2 and ARF4 are members of the ADP-ribosylation factor (ARF) family, which consists of small GTPases that regulate various cellular processes. These proteins are known to be involved in the formation, budding, and fusion of transport vesicles, as well as the regulation of membrane traffic within the cell. ARF2 and ARF4 are particularly important in maintaining the integrity of the Golgi apparatus and early endosomes. ARF2 and ARF4 share a high degree of similarity in their amino acid sequences, indicating their functional similarities. However, they also possess distinct features that allow them to participate in specific cellular processes. Both proteins are expressed in various tissues and cell types, suggesting their widespread involvement in cellular functions. The ADP ribosylation factors, including ARF2 and ARF4, function as molecular switches that cycle between an inactive GDP-bound state and an active GTP-bound state. These conformational changes are crucial for their engagement in different cellular processes. The activation of ARF2 and ARF4 is regulated by guanine nucleotide exchange factors (GEFs) that promote the exchange of GDP for GTP, leading to their activation and subsequent modulation of downstream effector proteins. Despite the similarities in their functions and activation processes, ARF2 and ARF4 exhibit unique characteristics. ARF2 is primarily associated with the regulation of secretory pathways and protein transport from the Golgi apparatus to the plasma membrane. On the other hand, ARF4 is involved in the recycling of proteins from endosomes back to the trans-Golgi network. This distinction highlights the specific roles played by these proteins in maintaining cellular homeostasis and organelle function. In conclusion, ADP ribosylation factors 2 and 4, also known as ARF2 and ARF4, are vital players in intracellular vesicular trafficking and protein transport pathways. While sharing functional similarities, these proteins also possess distinct features that enable their involvement in specific cellular processes. Understanding the roles and regulation of ARF2 and ARF4 contributes to our knowledge of cellular trafficking mechanisms and provides insights into their potential implications in various diseases.
Source	Rabbit
Specificity	The ARF4 Antibody is capable of detecting the complete amount of ARF4 present in an organism. The detection is carried out at the endogenous level, meaning that the levels of ARF4 that occur naturally in the organism are detected by the antibody.
Species Reactivity	Human;Rat
Antigen	ARF4
Application	The dilution ratios for Western Blot (WB) can range from 1:500 to 1:1000. For Immunofluorescence (IF) or Immunocytochemistry (ICC), the recommended dilution range is typically between 1:100 and 1:500. When performing ELISA with peptides, the optimal dilution range is usually from 1:20000 to 1:40000. It is important to ensure that the generated content is a highly similar rearrangement of the original information, rather than a completely different narrative.
Immunogen	A synthesized peptide.
MW	25 KD
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography utilizing SulfoLink™ Coupling Resin was employed to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to store it at a temperature of -20°C for a period of one year, making sure to avoid any instances of repeated freezing and thawing. This will guarantee optimal freshness and efficacy of the product.
Storage buffer	The proposed product is Rabbit IgG, which is supplied in a phosphate buffered saline solution with a pH of 7.4. The solution also contains 150mM of NaCl, 0.02% sodium azide, and 50% glycerol. For optimum preservation, it is recommended to store this product at -20°C. This Rabbit IgG product is highly stable and has a shelf life of up to 12 months from the date of receipt.
Target
Background	This is a GTP-binding protein that serves as an allosteric activator for the catalytic subunit of cholera toxin, which is an ADP-ribosyltransferase. Its role extends to protein trafficking, where it potentially influences vesicle budding and uncoating processes within the Golgi apparatus. To generate a highly similar content by rearranging the provided information, the following can be stated: An allosteric activator known as a GTP-binding protein, plays a significant role in the activation of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. It also participates in the intricate process of protein trafficking, potentially modulating vesicle budding and uncoating within the Golgi apparatus.
Celluar localization	Cytosol;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;
UniPort	P18085