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Rabbit Anti-TERT Polyclonal Antibody#abs131514

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Description

Catalog-specification	Delivery time	USD price
abs131514-100ug	1-2 Weeks	301.0
abs131514-50ug	1-2 Weeks	201.0

Overview
catalog	abs131514
Description	Telomerase is a crucial ribonucleoprotein enzyme that is involved in the replication of chromosome termini in the majority of eukaryotic organisms. While it is highly active in progenitor and cancer cells, its activity is usually very low, or nonexistent, in normal somatic cells. This enzyme is a catalytic component of the telomerase holoenzyme complex, which primarily works to elongate telomeres by operating as a reverse transcriptase, copying simple sequence repeats to the ends of chromosomes by replicating a template sequence found within the RNA component of the enzyme.
Other names	Below are rearranged versions of the given content: 1. Telomerase reverse transcriptase (TERT), also known as hTRT, TCS1, or Telomerase catalytic subunit, is a crucial protein associated with telomeres. 2. Telomerase-associated protein 2 (TP2), also referred to as Telomerase associated protein 2 or Telomerase Reverse Transcriptase, plays a significant role in telomere maintenance. 3. EST2 and HEST2 are two variants of Telomerase reverse transcriptase (TERT), which function in maintaining the integrity of telomeres. 4. The protein PFBMFT1, also called Telomerase-associated protein 2, acts in coordination with telomerase for maintaining the length of telomeres. 5. CMM9, DKCA2, and DKCB4 are gene codes associated with telomerase-related proteins involved in telomere maintenance. Please note that the rearranged versions maintain the original information while presenting it in a slightly different structure.
Source	Rabbit
Specificity	The TERT Antibody has the ability to detect the presence of total TERT in the body. It is designed to identify endogenous levels of this protein.
Species Reactivity	Human
Antigen	TERT
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human TERT.
MW	127 kDa
Properties
Concentration	1mg/ml
purification	The process of purifying the antiserum involved using SulfoLink™ Coupling Resin in peptide affinity chromatography. This technique offered a highly effective method of isolating the specific peptides in the antiserum. Through this process, a highly purified antiserum was obtained.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
Tissue specificity	Expressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T-lymphocytes, and at a low to undetectable level in peripheral blood T-lymphocytes.
Posttranslational modification	Phosphorylation at Tyr-707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation at Ser-227 by the AKT pathway promotes nuclear location. Phosphorylation at the G2/M phase at Ser-457 by DYRK2 promotes ubiquitination by the EDVP complex and degradation.Ubiquitinated by the EDVP complex, a E3 ligase complex following phosphorylation at Ser-457 by DYRK2. Ubiquitinated leads to proteasomal degradation. In case of infection by HIV-1, the EDVP complex is hijacked by HIV-1 via interaction between HIV-1 Vpr and DCAF1/VPRBP, leading to ubiquitination and degradation.
Celluar localization	Mitochondrion;Nucleus;Plasma Membrane;
UniPort	O14746