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Rabbit Anti-TARDBP Polyclonal Antibody#abs135837

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Description

Catalog-specification	Delivery time	USD price
abs135837-100ug	1-2 Weeks	301.0
abs135837-50ug	1-2 Weeks	201.0

Overview
catalog	abs135837
Description	TDP43, also known as TAR DNA-binding protein 43, plays a crucial role in regulating transcription and splicing of exons (1,2). In healthy conditions, TDP43 is predominantly found in the nucleus. However, in patients with frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS), there is an abnormal accumulation of TDP43 in insoluble aggregates. These aggregates are formed due to abnormal ubiquitination, phosphorylation, and cleavage of TDP43, resulting in the generation of carboxy-terminal fragments. These fragments get sequestered as insoluble aggregates in various parts of neurons, including nuclei, perikarya, and neurites (3,4). Furthermore, apart from its role in neurodegenerative disorders, TDP43 also has other important functions. It can inhibit the expression of the HIV-1 gene and regulate the splicing of the CFTR gene (1,5). This highlights the diverse and complex roles played by TDP43 in cellular processes and its significance beyond neurodegenerative diseases.
Other names	，。：ALS10；OTTHUMP00000002171；OTTHUMP00000002172；OTTHUMP00000002173；TADBP_HUMAN；TAR DNA43；TAR DNA；TAR DNA43；TARDBP；TDP 43；TDP-43；TDP43。
Source	Rabbit
Specificity	The TARDBP Antibody is designed to specifically detect the total TARDBP protein at its natural levels in the body.
Species Reactivity	Human;Mouse;Rat
Antigen	TARDBP
Application	The recommended dilutions for using this antibody in various assay types are as follows: for Western blotting, dilute the antibody at a range of 1:500 to 1:2000, for immunohistochemistry dilutions at 1:50 to 1:200 are recommended, and when using the antibody in ELISA experiments that involve peptides, dilute the antibody at a range of 1:20000 to 1:40000. It is important to follow the suggested dilutions in order to achieve optimal results when using this antibody in these assay types.
Immunogen	A synthesized peptide derived from human TARDBP.
MW	43kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography was used to purify the antiserum with the aid of SulfoLink™ Coupling Resin. The resin was utilized to couple peptides to a solid support, enabling the selective binding of the desired antiserum. The resulting purified antiserum was highly specific and effective in its intended application.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
Tissue specificity	Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
Posttranslational modification	Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Celluar localization	Nucleus;
UniPort	Q13148