Rabbit Anti-Acetyl-p53 (Lys317) Polyclonal Antibody#abs130619

abs130619-50ug

1-2 Weeks

201

abs130619-100ug

1-2 Weeks

301

Description

The nuclear protein tumor protein p53 plays a crucial role in regulating the cell cycle, with a specific focus on the transition from G0 to G1. Its presence is usually low within normal cells, but in various transformed cell lines, it is highly expressed, which contributes to the development of malignant cells.

Other names

p53, also known as Tumor Protein 53, TRP53, or Transformation-related Protein 53, is an important cellular tumor antigen. It plays a crucial role as a tumor suppressor and is encoded by the gene TP53. Mutations in the TP53 gene are associated with various types of cancer.
Another name for p53 is Cellular Tumor Antigen p53 or CTAG p53. This phosphoprotein is involved in regulating cell cycle progression, DNA repair, and apoptosis. Its function is vital for maintaining genetic stability and preventing the development of cancer.
p53 is also referred to as FLJ92943 or LFS1. This mutant tumor protein 53 is often found in mutated forms in cancer cells, leading to the loss of its tumor-suppressing abilities. However, the normal, non-mutated p53 acts as a guardian of the genome and prevents the proliferation of cells with damaged DNA.
Identified as BCC7 or Antigen NY-CO-13, p53 has gained significant attention in cancer research due to its pivotal role in controlling cell division and suppressing tumor formation. Its activity is regulated by post-translational modifications, such as phosphorylation, acetylation, and ubiquitination.
In summary, p53 is a multifunctional protein that serves as a critical tumor suppressor. Its disruption through mutations can contribute to the development and progression of various types of cancer. Understanding the mechanisms and functions of p53 is crucial for developing targeted therapies against cancer.

Source

Rabbit

Specificity

The acetyl-p53 (Lys317) Antibody specifically recognizes the presence of total p53 protein in its acetylated form at lysine317. This antibody is capable of detecting endogenous levels of p53 protein only when acetylated at lysine317. We can ensure accurate detection and analysis of p53 protein acetylation using this highly effective antibody.

Application

：：1:500~1:1000， (IHC)：1:50~1:500， (IF)：1:200， (ELISA) ()：1:20000-1:40000。

Immunogen

Properties

Concentration

Purification

affinity purification.

Clonality

Polyclonal Antibody

Stability & Storage

Storage buffer

Phosphate buffered saline with Rabbit IgG, pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol can be stored at -20 °C. It remains stable for 12 months starting from the date of receipt.

Target

Background

TP53, also known as p53, acts as a crucial tumor suppressor in various types of tumors. It plays a pivotal role in regulating cell growth and can either induce growth arrest or trigger apoptosis, depending on the specific circumstances and cell type. Its primary function involves controlling a subset of genes that are essential for cell division, thus negatively regulating this process. One of the genes activated by TP53 is an inhibitor of cyclin-dependent kinases, which are critical for cell cycle progression. Furthermore, TP53 can stimulate the expression of BAX and FAS antigens to promote apoptosis or suppress the expression of Bcl-2 to initiate programmed cell death.
Additionally, TP53 collaborates with mitochondrial PPIF to activate oxidative stress-induced necrosis, independent of transcriptional regulation. It also plays a role in transcriptional regulation by inducing the expression of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 contributes to TP53-dependent transcriptional repression, leading to apoptosis and having no effect on cell cycle regulation. TP53 is also involved in Notch signaling crossover.
In response to DNA damage, TP53 prevents cell cycle progression by inhibiting the activity of CDK7 kinase when associated with the CAK complex. Different isoforms of TP53 exert varying effects on its transactivation activity and growth suppression capabilities. For example, isoform 2 enhances the transactivation activity of isoform 1 from certain TP53-inducible promoters, while isoform 4 inhibits transactivation activity and impairs growth suppression mediated by isoform 1. Furthermore, isoform 7 inhibits isoform 1-mediated apoptosis.
Moreover, TP53 regulates the circadian clock by repressing the transcriptional activation of PER2 mediated by CLOCK-ARNTL/BMAL1.
Overall, TP53 plays a multifaceted role in tumor suppression, cell cycle regulation, apoptosis induction, and circadian rhythm modulation. Its activity is tightly regulated, and its dysregulation can have significant implications in various disease processes, particularly in cancer.

Tissue specificity

Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.

Celluar localization

Cytosol;Endoplasmic reticulum;Mitochondrion;Nucleus;

UniPort

P04637