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Rabbit Anti-Splicing Factor1 Polyclonal Antibody#abs140556

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Description

Catalog-specification	Delivery time	USD price
abs140556-100ug	1-2 Weeks	301.0
abs140556-50ug	1-2 Weeks	201.0

Overview
catalog	abs140556
Other names	The Zinc finger gene in MEN1 locus, also known as ZNF162, is an important protein that plays a crucial role in regulating splicing within mammalian cells. Another name for it is Mammalian branch point binding protein mBBP. It is also referred to as Splicing factor 1 and Transcription factor ZFM1. This protein is responsible for binding to important regions of RNA molecules called branch points, which are essential for proper splicing of precursor mRNA. It is encoded by the D11S636 gene with multiple names including OTTHUMP00000069749, OTTHUMP00000069750, and OTTHUMP00000069752. The precise role that ZNF162 plays in splicing is still being studied, but it has been shown to interact with other splicing factors to promote proper mRNA processing. It contains several zinc finger domains which are thought to play a role in its protein-protein interactions. This protein is essential for the proper functioning of cells, and mutations in the D11S636 gene have been linked to various diseases and disorders, including cancer. In summary, the Zinc finger gene in MEN1 locus, also known as ZNF162, is a critical protein that regulates splicing within mammalian cells. It binds to RNA branch points to facilitate proper mRNA processing and is encoded by the D11S636 gene. It plays a crucial role in the proper functioning of cells and is linked to various diseases and disorders.
Source	Rabbit
Specificity	The detection of total Splicing factor 1 at its endogenous levels can be achieved using the Splicing factor 1 Antibody.
Species Reactivity	Human
Antigen	Splicing factor1
Application	For Western blot, a dilution of 1:1000 is recommended. When performing immunofluorescence or immunocytochemistry, a dilution of 1:100-1:500 is appropriate. If you plan to use ELISA with a peptide substrate, a dilution of 1:20000-1:40000 is suggested. These dilutions have been tested and found to offer optimal results for these applications.
Immunogen	A peptide that has been synthesized from Splicing factor 1 of human origin, is what we are referring to. To generate similarly structured content, we need to ensure that it is based on the information provided in the original text.
MW	68kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, we purified the antiserum through peptide affinity chromatography. Our process resulted in a highly purified product that met our stringent quality standards.
Clonality	Polyclonal Antibody
Stability & Storage	One year of storage at -20 °C is recommended, with caution to avoid repeated freeze/thaw cycles. It is important to ensure the preservation of the product during storage for its proper usage and effectiveness.
Storage buffer	This product is Rabbit IgG in a solution of phosphate buffered saline at a pH level of 7.4. It also contains 150mM of NaCl, 0.02% sodium azide, and 50% glycerol. To preserve its quality, it's best to store it at -20°C. This solution is stable for a maximum of 12 months starting from the date of purchase.
Target
Background	Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
Tissue specificity	Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
Posttranslational modification	Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463.
Celluar localization	Nucleus;
UniPort	Q15637