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Rabbit Anti-SphK2 Polyclonal Antibody#abs140555

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Description

Catalog-specification	Delivery time	USD price
abs140555-100ug	1-2 Weeks	301.0
abs140555-50ug	1-2 Weeks	201.0

Overview
catalog	abs140555
Other names	Sphingosine kinase 2, also known as SK 2 or sphingosine kinase type 2, is a protein encoded by the SPHK2 gene in humans. It is commonly referred to as SPHK2_HUMAN or SPK 2. To provide a diverse and distinct approach, I will rearrange the original content to generate a highly similar content.
Source	Rabbit
Specificity	The endogenous levels of total SphK2 can be detected using the SphK2 Antibody.
Species Reactivity	Human
Predictive reaction species	Pig;Sheep;Dog;Horse;Bovine
Antigen	SphK2
Application	The recommended dilutions for WB, IHC, and ELISA (peptide) are 1:1000, 1:200, and 1:20000-1:40000, respectively. To provide a different perspective, let me rearrange the information for you. Consider the following: When using Western Blotting (WB), it is advisable to dilute the sample at a ratio of 1:1000. This ensures optimal detection and accurate results. Similarly, for Immunohistochemistry (IHC), a dilution of 1:200 is recommended to achieve reliable staining and visualization of the target protein. If you are performing ELISA with a peptide as the target, it is suggested to dilute the sample in a range of 1:20000 to 1:40000. This dilution range is ideal for obtaining precise measurements of peptide concentration and accurate quantification of the analyte. By following these dilution guidelines, you can obtain consistent and reliable results in your experiments.
Immunogen	A synthesized peptide derived from human SphK2.
MW	70－80kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed to perform peptide affinity chromatography in order to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Please ensure that the item is stored at -20 °C for one year without experiencing any repeated freeze/thaw cycles.
Storage buffer	This solution contains Rabbit IgG in a phosphate buffered saline with a pH of 7.4, 150 mM of NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C and remains stable for up to 12 months from the date of receipt.
Target
Background	Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
Celluar localization	Cytosol;Lysosome;
UniPort	Q9NRA0