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Rabbit Anti-SPHK1 Polyclonal Antibody#abs135525

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Description

Catalog-specification	Delivery time	USD price
abs135525-100ug	1-2 Weeks	301.0
abs135525-50ug	1-2 Weeks	201.0

Overview
catalog	abs135525
Other names	Sphingosine kinase 1, also known as SK1 or SPHK 1, is a protein encoded by the SPHK1_HUMAN gene. It plays a crucial role in the conversion of sphingosine to sphingosine-1-phosphate. SPK, another name for Sphingosine kinase 1, is involved in various cellular processes, including cell growth, survival, and migration. Understanding the functions and mechanisms of SK1 can provide valuable insights into its potential therapeutic applications.
Source	Rabbit
Specificity	The total levels of endogenous SPHK1 can be detected using the SPHK1 Antibody.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig
Antigen	SPHK1
Application	The recommended dilutions for WB are 1:500-1:2000, while for IHC, the suggested range is 1:50-1:200. If you are using ELISA with a peptide, the appropriate dilution range is 1:20000-1:40000. These dilutions have been carefully tested and optimized to ensure accurate and reliable results. When working with antibodies, it is essential to ensure that you use the correct dilution to obtain the best possible signal-to-noise ratio and to avoid non-specific binding. By following these recommendations, you can increase your chances of success in your experiments and make meaningful discoveries in your research.
Immunogen	A synthesized peptide derived from human SPHK1.
MW	43kDa
Properties
Concentration	1mg/ml
purification	Purification of the antiserum was carried out through the utilization of SulfoLink™ Coupling Resin in peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the substance to repeated freeze/thaw cycles in order to prevent any potential damage.
Storage buffer	The storage condition for Rabbit IgG is as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. Please note that it is stable for a period of 12 months from the date of receipt.
Target
Background	Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Tissue specificity	Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
Celluar localization	Cytosol;Nucleus;Plasma Membrane;
UniPort	Q9NYA1